Table 19.4 Coelenterazines and their properties.

Cat. No.
Coelenterazine Analog Em * (nm) RLC † Relative Intensity ‡ Half-Rise Time (msec) §
C2944 native 466 1.00 1 6–30
C14260 cp 442 0.63 28 2–5
C6779 f 472 0.80 20 6–30
C6780 h 466 0.75 16 6–30
* Emission maxima. † Relative luminescence capacity = total time-integrated emission of aequorin in saturating Ca2+ relative to native aequorin = 1.0. ‡ Relative intensity at 100 nM Ca2+. § Half-rise time = time for the luminescence signal to reach 50% of the maximum after addition of 1 mM Ca2+ to a standard of aequorin reconstituted with the coelenterazine analog of interest. All data are from Cell Calcium (1993) 14:373.
For Research Use Only. Not for use in diagnostic procedures.