Table 23.3 Contents of FocalCheck fluorescence microscope test slides.

FocalCheck fluorescence microscope test slide #1

Sample Area 1 Sample Area 2 Sample Area 3 Sample Area 4 Sample Area 5
Row A
15 µm 6 µm 4 µm 1 µm 0.5 µm
Green/orange/far-red thin rings Blue throughout/green ring Blue/green/red/far-red Blue/green/red/far-red Blue/green/red/far-red
Row B (Intensity Series)
6 µm 6 µm 6 µm 6 µm 6 µm
Green Green Green Green Green
100% intensity 33% intensity 10% intensity 3% intensity 0.67% intensity

FocalCheck fluorescence microscope test slide #2

Sample Area 1 Sample Area 2 Sample Area 3 Sample Area 4 Sample Area 5
Row A
6 µm 6 µm 6 µm 6 µm Mixture of all four double-stained beads
Green Orange Red Far-red
Bead 1 503/511 nm
Bead 2 511/524 nm
Bead 1 541/555 nm
Bead 2 545/565 nm
Bead 1 578/605 nm
Bead 2 589/613 nm
Bead 1 657/676 nm
Bead 2 671/692 nm
Row B (Intensity Series)
6 µm 6 µm 6 µm 6 µm Mixture of all eight reference beads
Green Orange Red Far-red
Ring 503/511 nm
Core 511/524 nm
Ring 541/555 nm
Core 545/565vnm
Ring 589/613 nm
Core 578/605 nm
Ring 671/692 nm
Core 657/676 nm

FocalCheck fluorescence microscope test slide #3

Sample Area 1 Sample Area 2 Sample Area 3 Sample Area 4 Sample Area 5
Row A
2.5 µm 2.5 µm 2.5 µm 2.5 µm 2.5 µm
Blue Green Orange Red Deep red
350/440 nm 505/515 nm 540/560 nm 580/605 nm 633/660 nm
Bright Bright Bright Bright Bright
100% intensity 100% intensity 100% intensity 100% intensity 100% intensity
Row B (Intensity Series)
2.5 µm 2.5 µm 2.5 µm 2.5 µm 2.5 µm
Blue Green Orange Red Deep red
350/440 nm 505/515 nm 540/560 nm 580/605 nm 633/660 nm
Dim Dim Dim Dim Dim
1% intensity 1% intensity 1% intensity 1% intensity 1% intensity
For Research Use Only. Not for use in diagnostic procedures.