Table 10.4 Detection limits of the EnzChek Protease Assay Kits.

Enzyme (Source) Class Detection Limit (Units) * Buffer Conditions
Elastase, Type IV (porcine pancreas)Serine protease2.2 × 10-310 mM Tris-HCl, pH 8.8
Chymotrypsin, Type II (bovine pancreas)Serine protease5.0 × 10-510 mM Tris-HCl, pH 7.8
Thermolysin (Bacillus thermoproteolyticus Rokko)Acid protease4.4 × 10-510 mM Tris-HCl, pH 7.8
Trypsin, Type IX (porcine pancreas)Serine protease1.3 × 10-210 mM Tris-HCl, pH 7.8
Papain (papaya latex)Cysteine protease2.1 × 10-410 mM MES, pH 6.2
Pepsin (porcine stomach mucosa)Acid protease2.1 × 10-310 mM HCl, pH 2.0
Elastase (Pseudomonas aeruginosa)Metallo-protease1.0 × 10-320 mM sodium phosphate, pH 8.0
Cathepsin DAcid protease2.0 × 10-420 mM sodium citrate, pH 5.0
Elastase (human leukocyte)Serine protease1.0 × 10-310 mM Tris-HCl, pH 7.5
* The detection limit is defined as the amount of enzyme required to cause a 10–20% change in fluorescence compared with the control sample at 22°C. Enzyme unit definitions are standard definitions for each individual enzyme. Detection limits were determined with BODIPY FL casein and with BODIPY TR-X casein; both substrates yielded similar results. Detection limits may vary with instrumentation.
For Research Use Only. Not for use in diagnostic procedures.