Fluorescent dyes successfully tested with the Image-iT FX signal enhancer—Table 7.8

Dyes * with potentially strong background fluorescence that is reduced with the Image-iT FX Signal Enhancer †
  • Fluorescein
  • Atto 610
  • Alexa Fluor 594
  • Oregon Green 488
  • Cascade Blue
  • Alexa Fluor 610
  • Oregon Green 514
  • Alexa Fluor 405
  • Alexa Fluor 633
  • Tetramethylrhodamine
  • Alexa Fluor 430
  • Alexa Fluor 635
  • Texas Red
  • Alexa Fluor 488
  • Alexa Fluor 647
  • Cascade Yellow
  • Alexa Fluor 500
  • Alexa Fluor 660
  • Dy 565
  • Alexa Fluor 514
  • Alexa Fluor 680
  • Dy 630
  • Alexa Fluor 555
  • Alexa Fluor 700
  • Atto 590
  • Alexa Fluor 568
  • Alexa Fluor 750
Dyes * with potentially weak background fluorescence that is reduced with the Image-iT FX Signal Enhancer †
  • Cy5
  • Alexa Fluor 532
  •  Allophycocyanin
  • Dy 635
  • Alexa Fluor 546
  •  R-phycoerythrin
  • Marina Blue
  
Background-free fluorescent dyes * that are not affected by the Image-iT FX Signal Enhancer ‡
  • Alexa Fluor 350
  • Pacific Blue
  • Texas Red-X
  • IRTM 790
  • Rhodamine B
  • Dy 550
  • Cy3
  • Rhodamine Red-X
  • Dy 610
* All dyes were conjugated to streptavidin and tested at 10 µg/mL. † Background staining was blocked by the Image-iT FX signal enhancer. ‡ Staining was unaffected by the Image-iT FX signal enhancer.
For Research Use Only. Not for use in diagnostic procedures.