Table 15.5 Fluorogenic substrates for caspase activity.

Peptide Sequence Target for Caspases *
Fluorophore †
R110 AMC
YVAD 1, 4 R33750  
VDVAD 2 R33755  
DEVD 3, 6, 7, 8, 10 E13184 ‡
R22120 §
R35100 **
E13183 ‡
VEID 6 R33754  
IETD 8, granzyme B R22125
R22126 §
 
LEED 13 R33753  
AAD granzyme B R33752  
Aspartic acid generic R22122  
* Caspase substrates can often be cleaved by multiple enzymes. The caspase most often associated with the given peptide sequence is listed first. † R110 = rhodamine 110; AMC = 7-amino-4-methylcoumarin. The absorption and emission maxima for the cleaved fluorophores at pH 7 are: 496/520 nm for R110 and 342/441 nm for AMC. ‡ EnzChek Caspase-3 Assay Kit. § Bulk packaging. ** RediPlate 96 EnzChek Caspase-3 Assay Kit.
For Research Use Only. Not for use in diagnostic procedures.