Table 3.1 Molecular Probes azide and alkyne derivatives.

Compound or Fluorophore Azide or Alkyne Cat. No. Application
Azide- or Alkyne-Modified Biomolecules for Metabolic or Enzymatic Labeling
EdU (5-ethynyl-2'-deoxyuridine)alkyneA10044
E10187
E10415
Detection of nascent DNA synthesis
EU (5-ethynyl uridine)alkyneE10345Detection of nascent RNA synthesis
Click-iT AHA (L-azidohomoalanine)azideC10102Detection of nascent protein synthesis or inhibition
Click-iT HPG (L-homopropargylglycine)alkyneC10186
Click-iT farnesyl alcohol, azideazideC10248Identification of isoprenylated proteins
Click-iT geranylgeranyl alcohol, azideazideC10249
Click-iT fucose alkyne (tetraacetylfucose alkyne)alkyneC10264Identification of fucosylated proteins
Click-iT LAA (linoleamide alkyne)alkyneC10447Identification of lipid peroxidation–derived protein modifications
Click-iT palmitic acid, azideazideC10265Identification of protein fatty acylation
Click-iT myristic acid, azideazideC10268
Click-iT GalNAz (tetraacetylated N-azidoacetylgalactosamine)azideC33365Identification of O-linked glycoproteins
Click-iT ManNAz (tetraacetylated N-azidoacetylmannosamine)azideC33366Identification of sialic acid–modified glycoproteins
Click-iT GlcNAz (tetraacetylated N-azidoacetylglucosamine)azideC33367Identification of O-linked glycoproteins
Azide- or Alkyne-Modified Fluorophores and Haptens for Detection (Ex/Em) *
Alexa Fluor 488 (495/519)azideA10266Fluorescent dye or hapten †
alkyneA10267
DIBO alkyneC10405Fluorescent dye (or hapten †) for copper-free labeling and detection of azide-modified targets
Alexa Fluor 555 (555/565)azideA20012Fluorescent dye
alkyneA20013
DIBO alkyneC10406Fluorescent dye for copper-free labeling and detection of azide-modified targets
Alexa Fluor 594 (590/617)azideA10270Fluorescent dye
alkyneA10275
DIBO alkyneC10407Fluorescent dye for copper-free labeling and detection of azide-modified targets
Alexa Fluor 647 (650/668)azideA10277Fluorescent dye
alkyneA10278
DIBO alkyneC10408Fluorescent dye for copper-free labeling and detection of azide-modified targets
Biotin (NA)‡azideB10184Hapten
alkyneB10185
DIBO alkyneC10412Hapten for copper-free labeling and detection of azide-modified targets
Oregon Green 488 (496/524)azideO10180Fluorescent dye or hapten †
alkyneO10181
Tetramethylrhodamine (TAMRA) (555/580)azideT10182Fluorescent dye or hapten †
alkyneT10183
DIBO alkyneC10410Fluorescent dye for copper-free labeling and detection of azide-modified targets
* Absorption and fluorescence emission maxima, in nm. † As hapten, use with anti-dye antibody; see Anti-Dye and Anti-Hapten Antibodies—Section 7.4. ‡ Requires streptavidin for detection; see Avidin, Streptavidin, NeutrAvidin and CaptAvidin Biotin-Binding Proteins and Affinity Matrices—Section 7.6.
For Research Use Only. Not for use in diagnostic procedures.