Parallel performance comparison of fluo-3 and fluo-4 on Molecular Devices FLIPR system—Table 19.3

Indicator µM * Time *(min)
Fluorescence †
Increase §
Basal Stimulated ‡
Fluo-3460170057003.4-fold
Fluo-44604900213004.3-fold
Fluo-4230120054004.5-fold
* CHO cells stably transfected with rat muscarinic M1 receptors were loaded with fluo-3 AM or fluo-4 AM according to standard protocols, with variations in indicator concentration (µM) and incubation time, as shown. † Relative fluorescence intensities after subtraction of background fluorescence. ‡ Cells were stimulated by addition of the muscarinic agonist carbachol (50 µM). § Ratio of stimulated to basal fluorescence intensities.
For Research Use Only. Not for use in diagnostic procedures.