Table 1.6 R0 values for some Alexa Fluor dyes.

Donor
Acceptor
Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 568 Alexa Fluor 594 Alexa Fluor 647
Alexa Fluor 350 50          
Alexa Fluor 488 NA 64 70 62 60 56
Alexa Fluor 546   NA   70 71 74
Alexa Fluor 555     NA   47 51
Alexa Fluor 568       NA   82
Alexa Fluor 594         NA 85
Alexa Fluor 647           NA
R0 values in angstroms (Å) represent the distance at which fluorescence resonance energy transfer from the donor dye to the acceptor dye is 50% efficient (Förster radius). Values were calculated from spectroscopic data as outlined (Fluorescence Resonance Energy Transfer (FRET)—Note 1.2). NA = Not applicable.
For Research Use Only. Not for use in diagnostic procedures.