Reactive oxygen species—Table 18.1

Reactive Oxygen Species Structure Detection Reagents
Hydrogen peroxideH2O2
Hydroxyl radical *HO•
  • 3'-(p-Aminophenyl) fluorescein (APF, A36003)
  • 3'-(p-Hydroxyphenyl) fluorescein (HPF, H36004)
  • CM-H2DCFDA (C6827) ref
  • Proxyl fluorescamine (C7924) ref
  • TEMPO-9-AC (A7923)
Hypochlorous acidHOCl
Nitric oxideNO
Peroxyl radical, including both alkylperoxyl and hydroperoxyl ref radicals (wherein R = H)ROO•
Peroxynitrite anion †ONOO
  • 3'-(p-Aminophenyl) fluorescein (APF, A36003)
  • 3'-(p-Hydroxyphenyl) fluorescein (HPF, H36004)
  • H2DCFDA (D399) ref
  • Carboxy-H2DCFDA (C400)
  • CM-H2DCFDA (C6827)
  • Coelenterazine (C2944) ref
  • Dihydrorhodamine 123 (D632, D23806) ref
  • Dihydrorhodamine 6G (D633)
  • Luminol (L8455) ref
Singlet oxygen ‡1O2
  • Singlet Oxygen Sensor Green reagent (S36002)
  • trans-1-(2'-methoxyvinyl)pyrene (M7913) ref
Superoxide anion•O2
* Hydroxyl radicals can also be photosensitized by malachite green isothiocyanate (M689) or generated by a N-(1,10-phenanthrolin-5-yl)iodoacetamide (P6879) metal–ligand complex. † 3-Nitrotyrosine, a product of this potent nitrating reagent, can be detected with an anti-nitrotyrosine antibody (A21285). ‡ Singlet oxygen can also be photosensitized by hypericin (H7476), rose bengal diacetate (R14000) and merocyanine 540 (M24571).
For Research Use Only. Not for use in diagnostic procedures.