Table 1.11 R0 values for QSY and dabcyl quenchers.

Donor
Acceptor
QSY 35 dabcyl QSY 7 and QSY 9 QSY 21
Alexa Fluor 350 47 50    
Alexa Fluor 488 44 49 64  
Alexa Fluor 546 25 29 67  
Alexa Fluor 555     45  
Alexa Fluor 568     56 75
Alexa Fluor 594       77
Alexa Fluor 647       69
R0 values in angstroms (Å) represent the distance at which fluorescence resonance energy transfer from the donor dye to the acceptor dye is 50% efficient (Förster radius). Values were calculated from spectroscopic data as outlined (see Fluorescence Resonance Energy Transfer (FRET)—Note 1.2).
For Research Use Only. Not for use in diagnostic procedures.