Selected haptenylation reagents and their anti-hapten antibodies—Table 4.2

Cat. No. Preferred Reactive Hapten(s) Unlabeled and Labeled Anti-Hapten Antibodies (Cat. No.) *
A30000
A30100
Alexa Fluor 405, SEAnti–Alexa Fluor 405/Cascade Blue dye (A5760)
A20000
A20100
A30005
A30052
Alexa Fluor 488, SE
Alexa Fluor 488, SE
Alexa Fluor 488, 5-TFP
Alexa Fluor 488, 5-SDP
Anti–Alexa Fluor 488 dye (A11094)
A29523-Amino-3-deoxydigoxigenin hemisuccinamide, SEAnti-digoxigenin (available from other suppliers)
B1582
B1606
B6353
B6352
Biotin-X, SE
Biotin-XX, SE
Biotin-X, SSE
Biotin-XX, SSE
Anti-biotin (03-3700)
D6102
B10006
BODIPY FL-X, SE
BODIPY FL, STP ester
Anti–BODIPY FL dye (A5770) †
C2284Cascade Blue acetyl azideAnti–Alexa Fluor 405/Cascade Blue dye (A5760)
D6104Dansyl-X, SEAnti-dansyl (A6398)
D2248
B2604
DNP-X, SE
DNP-X–biocytin-X, SE
Anti-DNP (A6423, A6430, A6435, A11097, Q17421)
F2181
F6130
Fluorescein 5(6)-SFX
Fluorescein-EX, SE
Anti–fluorescein/Oregon Green dye (A889, A982, A6413, A6421, A11090, A11091, A11095, A21253, Q15421, Q15431) †
L1338Lucifer yellow iodoacetamideAnti–lucifer yellow dye (A5750, A5751)
O6185Oregon Green 488-X, SEAnti–fluorescein/Oregon Green dye (A889, A982, A6413, A6421, A11090, A11091, A11095, A21253, Q15421, Q15431) †
T61055(6)-TAMRA-X, SEAnti-tetramethylrhodamine (A6397), anti–Texas Red dye (A6399) †
R6160Rhodamine Red-X, SEAnti-tetramethylrhodamine (A6397), anti–Texas Red dye (A6399) †
T6134
T20175
Texas Red-X, SEAnti-tetramethylrhodamine (A6397), anti–Texas Red dye (A6399) †
* See Anti-Dye and Anti-Hapten Antibodies—Section 7.4 for a description of these anti-hapten antibodies. † Both the anti-tetramethylrhodamine and the anti–Texas Red dye antibodies crossreact with tetramethylrhodamine, Lissamine rhodamine, Rhodamine Red and Texas Red fluorophores. Therefore, these fluorophores should not be used simultaneously to generate separate signals in a multicolor experiment. Similarly, the anti–BODIPY FL dye antibody may crossreact with other BODIPY dyes, and the anti–fluorescein/Oregon Green dye antibody crossreacts with both fluorescein and Oregon Green dyes.

 

For Research Use Only. Not for use in diagnostic procedures.