Table 23.1 Spectral characteristics of Molecular Probes dyes.

Dye Excitation Maximum (nm)* Emission Maximum (nm)*
Acridine orange spectra500 (+DNA)
460 (+RNA)
526 (+DNA)
650 (+RNA)
Alexa Fluor 350 dye, 7-amino-4-methylcoumarin (AMC) spectraspectra346442
Alexa Fluor 405 dye spectra402421
Alexa Fluor 430 dye spectra434539
Alexa Fluor 488 dye spectra495519
Alexa Fluor 514 dye spectra518540
Alexa Fluor 532 dye spectra531554
Alexa Fluor 546 dye spectra556573
Alexa Fluor 555 dye spectra555565
Alexa Fluor 568 dye spectra578603
Alexa Fluor 594 dye spectra590617
Alexa Fluor 610 dye spectra612628
Alexa Fluor 633 dye spectra632647
Alexa Fluor 635 dye spectra633647
Alexa Fluor 647 dye spectra650668
Alexa Fluor 660 dye spectra663690
Alexa Fluor 680 dye spectra679702
Alexa Fluor 700 dye spectra702723
Alexa Fluor 750 dye spectra749775
Alexa Fluor 790 dye spectra782805
Allophycocyanin spectra650660
7-Aminoactinomycin D (7-AAD) † spectra546647
BCECF indicator spectra482 (low pH)
503 (high pH)
520 (low pH)
528 (high pH)
Bimane380458
BOBO-1, BO-PRO-1 spectraspectra462481
BODIPY 630/650 dye spectra630650
BODIPY 650/665 dye spectra650665
BODIPY FL dye spectraspectra505513
BODIPY TMR-X dye spectraspectra542574
BODIPY TR-X dye spectraspectra589617
Calcein spectra494517
Calcium Crimson indicator spectra590615
Calcium Green indicators spectra506533
Calcium Orange indicator spectra549576
Carboxy SNARF indicators spectraspectra548 (low pH)
576 (high pH)
587 (low pH)
635 (high pH)
6-Carboxyrhodamine 6G spectra525555
Cascade Blue dye spectra400420
Cascade Yellow dye spectra402545
DAPI † spectra358461
Di-8-ANEPPS, Di-4-ANEPPS ‡spectra468635
DiA ‡ spectra456590
DiD (DiIC18(5)) spectra644665
DiI (DiIC18(3)) spectra549565
DiO (DiOC18(3)) spectra484501
DiR (DiIC18(7)) spectra750779
ELF 97 alcohol spectra345530
Eosin spectra524544
ER Tracker Blue-White DPX ‡ spectra372556
EthD-1 † spectra528617
Ethidium bromide † spectra518605
Fluo-3 indicator spectra506526
Fluo-4 indicator spectra494516
Fluorescein, FITC spectraspectraspectraspectra494518
FM 1-43, FM 1-43FX ‡ spectra479598
FM 4-64, FM 4-64FX ‡ spectra506750
Fura-2 indicator spectra363 (low [Ca2+])
335 (high [Ca2+])
512 (low [Ca2+])
505 (high [Ca2+])
Fura Red indicator spectra472 (low [Ca2+])
436 (high [Ca2+])
657 (low [Ca2+])
637 (high [Ca2+])
Hoechst 33258, Hoechst 33342 † spectra352461
7-Hydroxy-4-methylcoumarin spectra360455
Indo-1 indicator spectra346 (low [Ca2+])
330 (high [Ca2+])
475 (low [Ca2+])
401 (high [Ca2+])
JC-1 spectra498 (monomer)
593 (aggregate)
525 (monomer)
595 (aggregate)
JC-9506525 (monomer)
635 (aggregate)
JOE dye spectra525555
Lissamine rhodamine B570590
Lucifer yellow CH spectra428536
LysoSensor Blue DND-167 spectra374425
LysoSensor Green DND-153, DND-189 spectra442505
LysoSensor Yellow/Blue DND-160 (PDMPO) spectra384 (low pH)
329 (high pH)
540 (low pH)
440 (high pH)
LysoTracker Green spectra504511
LysoTracker Red spectra577592
Magnesium Green indicator spectra506531
Marina Blue dye spectra365460
MitoTracker Green FM spectra490516
MitoTracker Orange CMTMRos spectra551576
MitoTracker Red CMXRos spectra578599
Monobromobimane § spectra394490
NBD amines spectra465535
NeuroTrace 500/525 green-fluorescent Nissl stain † spectra500525
Nile red ‡ spectra549628
Oregon Green 488 dye and Oregon Green 488 BAPTA indicators spectra496524
Oregon Green 514 dye spectra511530
Pacific Blue dye spectra410455
Pacific Orange dye spectra400551
POPO-1, PO-PRO-1 † spectraspectra434456
Propidium iodide † spectra536617
Rhodamine 110 spectra496520
Rhodamine Red dye spectra570590
R-phycoerythrin spectra565575
Resorufin spectra571585
RH 414 ‡500635
Rhod-2 indicator spectra552578
Rhodamine Green dye spectra502527
Rhodamine 123 spectra507529
ROX dye spectra580605
Sodium Green indicator spectra507535
SYTO blue-fluorescent nucleic acid stains 40, 41, 42, 43 †428 ± 6454 ± 13
SYTO blue-fluorescent nucleic acid stains 44, 45 † spectra450 ± 5478 ± 7
SYTO green-fluorescent nucleic acid stains 12, 13, 16, 21, 23, 24 † spectra494 ± 6515 ± 7
SYTO green-fluorescent nucleic acid stains 11, 14, 15, 20, 22, 25 † spectra515 ± 7543 ± 13
SYTO orange-fluorescent nucleic acid stains 80, 81, 82, 83 †537 ± 7552 ± 8
SYTO orange-fluorescent nucleic acid stains 84, 85 †567583
SYTO red-fluorescent nucleic acid stains 17, 59, 61, 64 † spectra615 ± 15632 ± 13
SYTO red-fluorescent nucleic acid stains 60, 62, 63 †655 ± 3675 ± 3
SYTOX Blue nucleic acid stain † spectra445470
SYTOX Green nucleic acid stain † spectra504523
SYTOX Orange nucleic acid stain † spectra547570
Tetramethylrhodamine, Rhodamine B spectraspectraspectraspectra555580
Texas Red and Texas Red-X dye spectraspectra595615
TOTO-1, TO-PRO-1 † spectraspectra514533
TOTO-3, TO-PRO-3 † spectraspectra642660
X-rhod-1 indicator spectra580602
YOYO-1, YO-PRO-1 † spectraspectra491509
YOYO-3, YO-PRO-3 † spectraspectra612631
* Fluorescence excitation and emission maxima in nm are listed here, and fluorescence spectra are available at (www.lifetech.com/spectraviewer). Transmittance curves and other specific microscopy filter information can be found through microscope manufacturers or at filter manufacturer websites, including Chroma Technology Corp. (www.chroma.com), Omega Optical, Inc. (www.omegafilters.com) and  Semrock, Inc. (www.semrock.com). † Excitation and emission peaks listed for dye/nucleic acid complex. ‡ Excitation and emission peaks listed for lipid-bound dye. § Excitation and emission peaks listed for glutathione-conjugated dye.
For Research Use Only. Not for use in diagnostic procedures.