Table 11.2 Spectral characteristics of Molecular Probes actin-selective probes.

Cat. No. Actin-Selective Probe Ex/Em *
Approximate MW
F-Actin–Selective Probes
A22281 Alexa Fluor 350 phalloidin 346/446 † 1100
N354 NBD phallacidin 465/536 1040
A12379 Alexa Fluor 488 phalloidin 495/517 † 1320
F432 Fluorescein phalloidin 496/516 † 1175
O7466 Oregon Green 488 phalloidin 496/520 † 1180
B607 BODIPY FL phallacidin 505/512 1125
O7465 Oregon Green 514 phalloidin 511/528 † 1280
A22282 Alexa Fluor 532 phalloidin 528/555 † 1350
R415 Rhodamine phalloidin 540/565 † 1250
A22283 Alexa Fluor 546 phalloidin 554/570 † 1800
A34055 Alexa Fluor 555 phalloidin 555/565 † 1800
B3475 BODIPY 558/568 phalloidin 558/569 1115
A12380 Alexa Fluor 568 phalloidin 578/600 † 1590
A12381 Alexa Fluor 594 phalloidin 593/617 † 1620
T7471 Texas Red-X phalloidin 591/608 † 1490
A22284 Alexa Fluor 633 phalloidin 625/645 † 1900
A34054 Alexa Fluor 635 phalloidin 633/648 † 1900
B12382 BODIPY 650/665 phalloidin 647/661 1200
A22287 Alexa Fluor 647 phalloidin 649/666 † 1950
A22285 Alexa Fluor 660 phalloidin 661/689 † 1750
A22286 Alexa Fluor 680 phalloidin 677/699 † 1850
B7474 Biotin-XX phalloidin NA 1300
P3457 Phalloidin NA 790
G-Actin–Selective Probes
D12371 Alexa Fluor 488 DNase I 495/519 >31,000
D12372 Alexa Fluor 594 DNase I 590/617 >31,000
* Excitation (Ex) and emission (Em) maxima, in nm. Spectra of phallotoxins are either in aqueous buffer, pH 7–9 (denoted †) or in methanol. Spectra of DNase I conjugates are in aqueous buffer, pH 7–8. NA = Not applicable.
For Research Use Only. Not for use in diagnostic procedures.