Summary of Molecular Probes secondary antibody conjugates—Table 7.1

Antibody Host Table No.
Anti–mouse IgGGoatTable 7.1A
Anti–mouse IgMGoatTable 7.1J
Anti–rabbit IgGGoatTable 7.1B
Anti–rat IgGGoatTable 7.1C
Anti–rat IgMGoatTable 7.1J
Anti–human IgGGoatTable 7.1D
Anti–human IgMGoatTable 7.1J
Anti–chicken IgGGoatTable 7.1G
Anti–guinea pig IgGGoatTable 7.1H
Anti–hamster IgGGoatTable 7.1I
Anti–mouse IgGRabbitTable 7.1A
Anti–rat IgGRabbitTable 7.1C
Anti–human IgGRabbitTable 7.1D
Anti–goat IgGRabbitTable 7.1F
Anti–mouse IgGChickenTable 7.1A
Anti–rabbit IgGChickenTable 7.1B
Anti–rat IgGChickenTable 7.1C
Anti–human IgGChickenTable 7.1D
Anti–goat IgGChickenTable 7.1F
Anti–mouse IgGDonkeyTable 7.1A
Anti–rabbit IgGDonkeyTable 7.1B
Anti–rat IgGDonkeyTable 7.1C
Anti–sheep IgGDonkeyTable 7.1E
Anti–goat IgGDonkeyTable 7.1F

Table 7.1A Molecular Probes affinity-purified anti–mouse IgG antibody conjugates

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Mouse IgG
A11045Alexa Fluor 350Blue346442
M10991Marina BlueBlue365460
C962Cascade BlueBlue400420
A31553Alexa Fluor 405Blue402421
P10993Pacific BlueBlue410455
A11063Alexa Fluor 430Yellow-green434539
F2761FluoresceinGreen494518
A11001Alexa Fluor 488Green495519
O6380Oregon Green 488Green496524
B2752BODIPY FLGreen505513
O6383Oregon Green 514Green511530
A31555Alexa Fluor 514Yellow-green518540
A11002Alexa Fluor 532Yellow531554
A10521Cy3Orange552570
T2762TetramethylrhodamineOrange555580
A11003Alexa Fluor 546Orange556573
A21422Alexa Fluor 555Orange555565
P852R-phycoerythrin (R-PE)Orange496, 546, 565578
A20980Alexa Fluor 610–R-PERed496, 546, 565628
A20990Alexa Fluor 647–R-PEFar-red496, 546, 565668
A20983Alexa Fluor 680–R-PEFar-red496, 546, 565702
R6393Rhodamine Red-XRed-orange570590
A11004Alexa Fluor 568Red-orange578603
A11005Alexa Fluor 594Red590617
T862Texas RedRed595615
T6390Texas Red-XRed595615
A21050Alexa Fluor 633Far-red632647
A31574Alexa Fluor 635Far-red633647
A10524Cy5Far-red649670
A865AllophycocyaninFar-red650660
A21000Alexa Fluor 680–allophycocyaninFar-red650702
A21006Alexa Fluor 750–allophycocyaninInfrared650775
A21235Alexa Fluor 647Far-red650668
A21054Alexa Fluor 660Far-red663690
A21057Alexa Fluor 680Far-red679702
A21036Alexa Fluor 700Infrared702723
A21037Alexa Fluor 750Infrared749775
A11375Alexa Fluor 790Infrared782805
B2763BiotinNA<300NA
G21060Alkaline phosphataseNANANA
G21040Horseradish peroxidaseNANANA
G31567β-LactamaseNANANA
A10535UnlabeledNANANA
Goat Anti–Mouse IgG, Highly Cross-Adsorbed
A21049Alexa Fluor 305Blue346442
P31582Pacific BlueBlue410455
A11029Alexa Fluor 488Green495519
O11033Oregon Green 488Green496524
A11030Alexa Fluor 546Orange556573
A21424Alexa Fluor 555Orange555565
A11031Alexa Fluor 568Red-orange578603
A11032Alexa Fluor 594Red590617
A21052Alexa Fluor 633Far-red632647
A31575Alexa Fluor 635Far-red633647
A21236Alexa Fluor 647Far-red650668
A21055Alexa Fluor 660Far-red663690
A21058Alexa Fluor 680Far-red679702
A11357Alexa Fluor 790Infrared782805
D20691DSB-X biotinNA<300NA
Goat Anti–Mouse IgG, F(ab')2 Fragments
A11068Alexa Fluor 350Blue346442
P31585Pacific OrangeOrange400551
P31581Pacific BlueBlue410455
F11021FluoresceinGreen494518
A11017Alexa Fluor 488Green495519
A11018Alexa Fluor 546Orange556573
A21425Alexa Fluor 555Orange555565
A10543R-phycoerythrin (R-PE)Orange496, 546, 565578
A11019Alexa Fluor 568Red-orange578603
A11020Alexa Fluor 594Red590617
A21053Alexa Fluor 633Far-red632647
A10539AllophycocyaninFar-red650660
A21237Alexa Fluor 647Far-red650668
A21059Alexa Fluor 680Far-red679702
B11027BiotinNA<300NA
F21452Alkaline phosphataseNANANA
F21453Horseradish peroxidaseNANANA
A10534UnlabeledNANANA
Goat Anti–Mouse IgG, IgM
A10679FluoresceinGreen494518
A10680Alexa Fluor 488Green495519
A10677Horseradish peroxidaseNANANA
Goat Anti–Mouse IgG, IgM; F(ab')2 Fragments
A10683FluoresceinGreen494518
A10684Alexa Fluor 488Green495519
Goat Anti–Mouse IgG, IgM, IgA
A10667Alexa Fluor 488Green495519
A10676BiotinNA<300NA
A10668Horseradish peroxidaseNANANA
A10666UnlabeledNANANA
Rabbit Anti–Mouse IgG
A21062Alexa Fluor 350Blue346442
A11059Alexa Fluor 488Green495519
A11060Alexa Fluor 546Orange556573
A21427Alexa Fluor 555Orange555565
A11061Alexa Fluor 568Red-orange578603
A11062Alexa Fluor 594Red590617
A21063Alexa Fluor 633Far-red632647
A21239Alexa Fluor 647Far-red650668
A21065Alexa Fluor 680Far-red679702
R21455Horseradish peroxidaseNANANA
Rabbit Anti–Mouse IgG, F(ab')2 Fragments
A21204Alexa Fluor 488Green495519
A21205Alexa Fluor 594Red590617
Chicken Anti–Mouse IgG
A21200Alexa Fluor 488Green495519
A21201Alexa Fluor 594Red590617
A21463Alexa Fluor 647Far-red650668
Donkey Anti–Mouse IgG
A10035Alexa Fluor 350Blue346442
A21202Alexa Fluor 488Green495519
A10036Alexa Fluor 546Orange556573
A31570Alexa Fluor 555Orange555565
A10037Alexa Fluor 568Red-orange578603
A21203Alexa Fluor 594Red590617
A31571Alexa Fluor 647Far-red650668
A10038Alexa Fluor 680Far-red679702
A11371Alexa Fluor 790Infrared782805
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1B Molecular Probes affinity-purified anti–rabbit IgG antibody conjugates

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Rabbit IgG
A11046Alexa Fluor 350Blue346442
M10992Marina BlueBlue365460
C2764Cascade BlueBlue400420
P31584Pacific OrangeOrange400551
A31556Alexa Fluor 405Blue402421
P10994Pacific BlueBlue410455
A11064Alexa Fluor 430Yellow-green434539
F2765FluoresceinGreen494518
A11008Alexa Fluor 488Green495519
O6381Oregon Green 488Green496524
B2766BODIPY FLGreen505513
A31558Alexa Fluor 514Yellow-green518540
A11009Alexa Fluor 532Yellow531554
A10520Cy3Orange552570
T2769TetramethylrhodamineOrange555580
A11010Alexa Fluor 546Orange556573
A21428Alexa Fluor 555Orange555565
P2771MPR-phycoerythrin (R-PE)Orange496, 546, 565578
A20981Alexa Fluor 610–R-PERed496, 546, 565628
A20991Alexa Fluor 647–R-PEFar-red496, 546, 565668
A20984Alexa Fluor 680–R-PEFar-red496, 546, 565702
R6394Rhodamine Red-XRed-orange570590
A11011Alexa Fluor 568Red-orange578603
A11012Alexa Fluor 594Red590617
T2767Texas RedRed595615
T6391Texas Red-XRed595615
A21070Alexa Fluor 633Far-red632647
A31576Alexa Fluor 635Far-red633647
A10523Cy5Far-red649670
A10931AllophycocyaninFar-red650660
A21001MPAlexa Fluor 680–allophycocyaninFar-red650702
A21244Alexa Fluor 647Far-red650668
A21073Alexa Fluor 660Far-red663690
A21076Alexa Fluor 680Far-red679702
A21038Alexa Fluor 700Infrared702723
A21039Alexa Fluor 750Infrared749775
A11367Alexa Fluor 790Infrared782805
B2770BiotinNA<300NA
G21079Alkaline phosphataseNANANA
G21234Horseradish peroxidaseNANANA
G31568β-LactamaseNANANA
A10533UnlabeledNANANA
Goat Anti–Rabbit IgG, Highly Cross-Adsorbed
A21068Alexa Fluor 350Blue346442
A11034Alexa Fluor 488Green495519
O11038Oregon Green 488Green496524
A11035Alexa Fluor 546Orange556573
A21429Alexa Fluor 555Orange555565
A11036Alexa Fluor 568Red-orange578603
A11037Alexa Fluor 594Red590617
A21071Alexa Fluor 633Far-red632647
A31577Alexa Fluor 635Far-red633647
A21245Alexa Fluor 647Far-red650668
A21074Alexa Fluor 660Far-red663690
A21109Alexa Fluor 680Far-red679702
A11369Alexa Fluor 790Infrared782805
Goat Anti–Rabbit IgG, F(ab')2 Fragments
A11069Alexa Fluor 350Blue346442
A10526FluoresceinGreen494518
A11070Alexa Fluor 488Green495519
A11071Alexa Fluor 546Orange556573
A21430Alexa Fluor 555Orange555565
A10542R-phycoerythrin (R-PE)Orange496, 546, 565578
A21069Alexa Fluor 568Red-orange578603
A11072Alexa Fluor 594Red590617
A21072Alexa Fluor 633Far-red632647
A21246Alexa Fluor 647Far-red650668
A21077Alexa Fluor 680Far-red679702
B21078BiotinNA<300NA
F21456Alkaline phosphataseNANANA
A10547Horseradish peroxidaseNANANA
Chicken Anti–Rabbit IgG
A21441Alexa Fluor 488Green495519
A21442Alexa Fluor 594Red590617
A21443Alexa Fluor 647Far-red650668
Donkey Anti–Rabbit IgG
A10039Alexa Fluor 350Blue346442
A21206Alexa Fluor 488Green495519
A10040Alexa Fluor 546Orange556573
A31572Alexa Fluor 555Orange555565
A10042Alexa Fluor 568Red-orange578603
A21207Alexa Fluor 594Red590617
A31573Alexa Fluor 647Far-red650668
A10043Alexa Fluor 680Far-red679702
A11374Alexa Fluor 790Infrared782805
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1C Molecular Probes affinity-purified anti–rat IgG antibody conjugates

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Rat IgG
A21093Alexa Fluor 350Blue346442
A10528FluoresceinGreen494518
A11006Alexa Fluor 488Green495519
O6382Oregon Green 488Green496524
A10522Cy3Orange552570
A10531TetramethylrhodamineOrange555580
A11081Alexa Fluor 546Orange556573
A21434Alexa Fluor 555Orange555565
A10545R-phycoerythrin (R-PE)Orange496, 546, 565578
A11077Alexa Fluor 568Red-orange578603
A11007Alexa Fluor 594Red590617
T6392Texas Red-XRed595615
A21094Alexa Fluor 633Far-red632647
A10525Cy5Far-red649670
A10540AllophycocyaninFar-red650660
A21247Alexa Fluor 647Far-red650668
A21096Alexa Fluor 680Far-red679702
A10517BiotinNA<300NA
D20697DSB-X biotinNA<300NA
A10546Alkaline phosphataseNANANA
A10549Horseradish peroxidaseNANANA
A10536UnlabeledNANANA
Goat Anti–Rat IgG, F(ab')2 Fragments
A10527FluoresceinGreen494518
A10544R-phycoerythrin (R-PE)Orange496, 546, 565578
A10691Cy5–R-PEFar-red496, 546, 565670
A10548Horseradish peroxidaseNANANA
Rabbit Anti–Rat IgG
A21210Alexa Fluor 488Green495519
A21211Alexa Fluor 594Red590617
Chicken Anti–Rat IgG
A21470Alexa Fluor 488Green495519
A21471Alexa Fluor 594Red590617
A21472Alexa Fluor 647Far-red650668
Donkey Anti–Rat IgG
A21208Alexa Fluor 488Green495519
A21209Alexa Fluor 594Red590617
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1D Molecular Probes affinity-purified anti–human IgG antibody conjugates

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Human IgG
A11013Alexa Fluor 488Green495519
A21089Alexa Fluor 546Orange556573
A21433Alexa Fluor 555Orange555565
A21090Alexa Fluor 568Red-orange578603
A11014Alexa Fluor 594Red590617
A21091Alexa Fluor 633Far-red632647
A21445Alexa Fluor 647Far-red650668
Mouse Anti–Human IgG1
A10631Alexa Fluor 488Green495519
A10650BiotinNA<300NA
A10648Horseradish peroxidaseNANANA
A10630UnlabeledNANANA
Mouse Anti–Human IgG4
A10663BiotinNA<300NA
A10654Horseradish peroxidaseNANANA
A10651UnlabeledNANANA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1E Molecular Probes affinity-purified anti–sheep IgG antibody conjugates

Cat. No. Label Emission Color Abs * Em *
Donkey Anti–Sheep IgG
A21097Alexa Fluor 350Blue346442
A11015Alexa Fluor 488Green495519
A21098Alexa Fluor 546Orange556573
A21436Alexa Fluor 555Orange555565
A21099Alexa Fluor 568Red-orange578603
A11016Alexa Fluor 594Red590617
A21100Alexa Fluor 633Far-red632647
A21448Alexa Fluor 647Far-red650668
A21102Alexa Fluor 680Far-red679702
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm.

Table 7.1F Molecular Probes affinity-purified anti–goat IgG antibody conjugates

Cat. No. Label Emission Color Abs * Em *
Rabbit Anti–Goat IgG
A10529FluoresceinGreen494518
A11078Alexa Fluor 488Green495519
A10532TetramethylrhodamineOrange555580
A21085Alexa Fluor 546Orange556573
A21431Alexa Fluor 555Orange555565
A11079Alexa Fluor 568Red-orange578603
A11080Alexa Fluor 594Red590617
A21086Alexa Fluor 633Far-red632647
A21446Alexa Fluor 647Far-red650668
A21088Alexa Fluor 680Far-red679702
A10518BiotinNA<300NA
R21458Alkaline phosphataseNANANA
R21459Horseradish peroxidaseNANANA
A10537UnlabeledNANANA
Rabbit Anti–Goat IgG, F(ab')2 Fragments
A21222Alexa Fluor 488Green495519
A21223Alexa Fluor 594Red590617
Chicken Anti–Goat IgG
A21467Alexa Fluor 488Green495519
A21468Alexa Fluor 594Red590617
A21469Alexa Fluor 647Far-red650668
Donkey Anti–Goat IgG
A21081Alexa Fluor 350Blue346442
A11055Alexa Fluor 488Green495519
A11056Alexa Fluor 546Orange556573
A21432Alexa Fluor 555Orange555565
A11057Alexa Fluor 568Red-orange578603
A11058Alexa Fluor 594Red590617
A21082Alexa Fluor 633Far-red632647
A21447Alexa Fluor 647Far-red650668
A21083Alexa Fluor 660Far-red663690
A21084Alexa Fluor 680Far-red679702
A11370Alexa Fluor 790Infrared782805
D20698DSB-X biotinNA<300NA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1G Molecular Probes affinity-purified anti–chicken IgG antibody conjugates

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Chicken IgG
A11039Alexa Fluor 488Green495519
A11040Alexa Fluor 546Orange556573
A21437Alexa Fluor 555Orange555565
A11041Alexa Fluor 568Red-orange578603
A11042Alexa Fluor 594Red590617
A21103Alexa Fluor 633Far-red632647
A21449Alexa Fluor 647Far-red650668
D20701DSB-X biotinNA<300NA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1H Molecular Probes affinity-purified anti–guinea pig IgG antibody conjugates

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Guinea Pig IgG
A11073Alexa Fluor 488Green495519
A11074Alexa Fluor 546Orange556573
A21435Alexa Fluor 555Orange555565
A11075Alexa Fluor 568Red-orange578603
A11076Alexa Fluor 594Red590617
A21105Alexa Fluor 633Far-red632647
A21450Alexa Fluor 647Far-red650668
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates in nm.

Table 7.1I Molecular Probes affinity-purified goat anti–hamster IgG antibody conjugates

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Hamster IgG
A21110Alexa Fluor 488Green495519
A21111Alexa Fluor 546Orange556573
A21112Alexa Fluor 568Red-orange578603
A21113Alexa Fluor 594Red590617
A21451Alexa Fluor 647Far-red650668
* Approximate absorption (Abs) and emission (Em) maxima of conjugates, in nm.

Table 7.1J Molecular Probes affinity-purified goat anti–IgM (µ chain) antibody conjugates

Fluorophore Abs/Em * Anti–Mouse IgM † Anti–Rat IgM † Anti–Human IgM †
Alexa Fluor 350346/442A31552  
Alexa Fluor 488495/519A21042A21212A21215
Alexa Fluor 546556/573A21045  
Alexa Fluor 555555/565A21426  
R-phycoerythrin496, 546, 565/578A10689
A10690 ‡
  
Alexa Fluor 568578/603A21043  
Alexa Fluor 594590/617A21044A21213A21216
Alexa Fluor 633632/647A21046  
Allophycocyanin650/660A10688  
Alexa Fluor 647650/668A21238A21248A21249
Alexa Fluor 680679/702A21048  
DSB-X biotinNAD20693  
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. † May also react with IgM from other species. ‡ F(ab')2 fragments. NA = Not applicable.
For Research Use Only. Not for use in diagnostic procedures.