Table 7.1 Summary of Molecular Probes secondary antibody conjugates.

Antibody Host Table No.
Anti–mouse IgGGoatTable 7.1A
Anti–mouse IgMGoatTable 7.1J
Anti–rabbit IgGGoatTable 7.1B
Anti–rat IgGGoatTable 7.1C
Anti–rat IgMGoatTable 7.1J
Anti–human IgGGoatTable 7.1D
Anti–human IgMGoatTable 7.1J
Anti–chicken IgGGoatTable 7.1G
Anti–guinea pig IgGGoatTable 7.1H
Anti–hamster IgGGoatTable 7.1I
Anti–mouse IgGRabbitTable 7.1A
Anti–rat IgGRabbitTable 7.1C
Anti–human IgGRabbitTable 7.1D
Anti–goat IgGRabbitTable 7.1F
Anti–mouse IgGChickenTable 7.1A
Anti–rabbit IgGChickenTable 7.1B
Anti–rat IgGChickenTable 7.1C
Anti–human IgGChickenTable 7.1D
Anti–goat IgGChickenTable 7.1F
Anti–mouse IgGDonkeyTable 7.1A
Anti–rabbit IgGDonkeyTable 7.1B
Anti–rat IgGDonkeyTable 7.1C
Anti–sheep IgGDonkeyTable 7.1E
Anti–goat IgGDonkeyTable 7.1F

Table 7.1A Molecular Probes affinity-purified anti–mouse IgG antibody conjugates.

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Mouse IgG
A11045 Alexa Fluor 350 Blue 346 442
M10991 Marina Blue Blue 365 460
C962 Cascade Blue Blue 400 420
A31553 Alexa Fluor 405 Blue 402 421
P10993 Pacific Blue Blue 410 455
A11063 Alexa Fluor 430 Yellow-green 434 539
F2761 Fluorescein Green 494 518
A11001 Alexa Fluor 488 Green 495 519
O6380 Oregon Green 488 Green 496 524
B2752 BODIPY FL Green 505 513
O6383 Oregon Green 514 Green 511 530
A31555 Alexa Fluor 514 Yellow-green 518 540
A11002 Alexa Fluor 532 Yellow 531 554
A10521 Cy3 Orange 552 570
T2762 Tetramethylrhodamine Orange 555 580
A11003 Alexa Fluor 546 Orange 556 573
A21422 Alexa Fluor 555 Orange 555 565
P852 R-phycoerythrin (R-PE) Orange 496, 546, 565 578
A20980 Alexa Fluor 610–R-PE Red 496, 546, 565 628
A20990 Alexa Fluor 647–R-PE Far-red 496, 546, 565 668
A20983 Alexa Fluor 680–R-PE Far-red 496, 546, 565 702
R6393 Rhodamine Red-X Red-orange 570 590
A11004 Alexa Fluor 568 Red-orange 578 603
A11005 Alexa Fluor 594 Red 590 617
T862 Texas Red Red 595 615
T6390 Texas Red-X Red 595 615
A21050 Alexa Fluor 633 Far-red 632 647
A31574 Alexa Fluor 635 Far-red 633 647
A10524 Cy5 Far-red 649 670
A865 Allophycocyanin Far-red 650 660
A21000 Alexa Fluor 680–allophycocyanin Far-red 650 702
A21006 Alexa Fluor 750–allophycocyanin Infrared 650 775
A21235 Alexa Fluor 647 Far-red 650 668
A21054 Alexa Fluor 660 Far-red 663 690
A21057 Alexa Fluor 680 Far-red 679 702
A21036 Alexa Fluor 700 Infrared 702 723
A21037 Alexa Fluor 750 Infrared 749 775
A11375 Alexa Fluor 790 Infrared 782 805
B2763 Biotin NA <300 NA
G21060 Alkaline phosphatase NA NA NA
G21040 Horseradish peroxidase NA NA NA
G31567 β-Lactamase NA NA NA
A10535 Unlabeled NA NA NA
Goat Anti–Mouse IgG, Highly Cross-Adsorbed
A21049 Alexa Fluor 305 Blue 346 442
P31582 Pacific Blue Blue 410 455
A11029
R37120 †
Alexa Fluor 488 Green 495 519
O11033 Oregon Green 488 Green 496 524
A11030 Alexa Fluor 546 Orange 556 573
A21424 Alexa Fluor 555 Orange 555 565
A11031 Alexa Fluor 568 Red-orange 578 603
A11032
R37121 †
Alexa Fluor 594 Red 590 617
A21052 Alexa Fluor 633 Far-red 632 647
A31575 Alexa Fluor 635 Far-red 633 647
A21236 Alexa Fluor 647 Far-red 650 668
A21055 Alexa Fluor 660 Far-red 663 690
A21058 Alexa Fluor 680 Far-red 679 702
A11357 Alexa Fluor 790 Infrared 782 805
D20691 DSB-X biotin NA <300 NA
Goat Anti–Mouse IgG, F(ab')2 Fragments
A11068 Alexa Fluor 350 Blue 346 442
P31585 Pacific Orange Orange 400 551
P31581 Pacific Blue Blue 410 455
F11021 Fluorescein Green 494 518
A11017 Alexa Fluor 488 Green 495 519
A11018 Alexa Fluor 546 Orange 556 573
A21425 Alexa Fluor 555 Orange 555 565
A10543 R-phycoerythrin (R-PE) Orange 496, 546, 565 578
A11019 Alexa Fluor 568 Red-orange 578 603
A11020 Alexa Fluor 594 Red 590 617
A21053 Alexa Fluor 633 Far-red 632 647
A10539 Allophycocyanin Far-red 650 660
A21237 Alexa Fluor 647 Far-red 650 668
A21059 Alexa Fluor 680 Far-red 679 702
B11027 Biotin NA <300 NA
F21452 Alkaline phosphatase NA NA NA
F21453 Horseradish peroxidase NA NA NA
A10534 Unlabeled NA NA NA
Goat Anti–Mouse IgG, IgM
A10679 Fluorescein Green 494 518
A10680 Alexa Fluor 488 Green 495 519
A10677 Horseradish peroxidase NA NA NA
Goat Anti–Mouse IgG, IgM; F(ab')2 Fragments
A10683 Fluorescein Green 494 518
A10684 Alexa Fluor 488 Green 495 519
Goat Anti–Mouse IgG, IgM, IgA
A10667 Alexa Fluor 488 Green 495 519
A10676 Biotin NA <300 NA
A10668 Horseradish peroxidase NA NA NA
A10666 Unlabeled NA NA NA
Rabbit Anti–Mouse IgG
A21062 Alexa Fluor 350 Blue 346 442
A11059 Alexa Fluor 488 Green 495 519
A11060 Alexa Fluor 546 Orange 556 573
A21427 Alexa Fluor 555 Orange 555 565
A11061 Alexa Fluor 568 Red-orange 578 603
A11062 Alexa Fluor 594 Red 590 617
A21063 Alexa Fluor 633 Far-red 632 647
A21239 Alexa Fluor 647 Far-red 650 668
A21065 Alexa Fluor 680 Far-red 679 702
R21455 Horseradish peroxidase NA NA NA
Rabbit Anti–Mouse IgG, F(ab')2 Fragments
A21204 Alexa Fluor 488 Green 495 519
A21205 Alexa Fluor 594 Red 590 617
Chicken Anti–Mouse IgG
A21200 Alexa Fluor 488 Green 495 519
A21201 Alexa Fluor 594 Red 590 617
A21463 Alexa Fluor 647 Far-red 650 668
Donkey Anti–Mouse IgG
A10035 Alexa Fluor 350 Blue 346 442
A21202
R37114 †
Alexa Fluor 488 Green 495 519
A10036 Alexa Fluor 546 Orange 556 573
A31570 Alexa Fluor 555 Orange 555 565
A10037 Alexa Fluor 568 Red-orange 578 603
A21203
R37115 †
Alexa Fluor 594 Red 590 617
A31571 Alexa Fluor 647 Far-red 650 668
A10038 Alexa Fluor 680 Far-red 679 702
A11371 Alexa Fluor 790 Infrared 782 805
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. † ReadyProbes ready-to-use imaging reagent. NA = Not applicable.

Table 7.1B Molecular Probes affinity-purified anti–rabbit IgG antibody conjugates.

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Rabbit IgG
A11046 Alexa Fluor 350 Blue 346 442
M10992 Marina Blue Blue 365 460
C2764 Cascade Blue Blue 400 420
P31584 Pacific Orange Orange 400 551
A31556 Alexa Fluor 405 Blue 402 421
P10994 Pacific Blue Blue 410 455
A11064 Alexa Fluor 430 Yellow-green 434 539
F2765 Fluorescein Green 494 518
A11008 Alexa Fluor 488 Green 495 519
O6381 Oregon Green 488 Green 496 524
B2766 BODIPY FL Green 505 513
A31558 Alexa Fluor 514 Yellow-green 518 540
A11009 Alexa Fluor 532 Yellow 531 554
A10520 Cy3 Orange 552 570
T2769 Tetramethylrhodamine Orange 555 580
A11010 Alexa Fluor 546 Orange 556 573
A21428 Alexa Fluor 555 Orange 555 565
P2771MP R-phycoerythrin (R-PE) Orange 496, 546, 565 578
A20981 Alexa Fluor 610–R-PE Red 496, 546, 565 628
A20991 Alexa Fluor 647–R-PE Far-red 496, 546, 565 668
A20984 Alexa Fluor 680–R-PE Far-red 496, 546, 565 702
R6394 Rhodamine Red-X Red-orange 570 590
A11011 Alexa Fluor 568 Red-orange 578 603
A11012 Alexa Fluor 594 Red 590 617
T2767 Texas Red Red 595 615
T6391 Texas Red-X Red 595 615
A21070 Alexa Fluor 633 Far-red 632 647
A31576 Alexa Fluor 635 Far-red 633 647
A10523 Cy5 Far-red 649 670
A10931 Allophycocyanin Far-red 650 660
A21001MP Alexa Fluor 680–allophycocyanin Far-red 650 702
A21244 Alexa Fluor 647 Far-red 650 668
A21073 Alexa Fluor 660 Far-red 663 690
A21076 Alexa Fluor 680 Far-red 679 702
A21038 Alexa Fluor 700 Infrared 702 723
A21039 Alexa Fluor 750 Infrared 749 775
A11367 Alexa Fluor 790 Infrared 782 805
B2770 Biotin NA <300 NA
G21079 Alkaline phosphatase NA NA NA
G21234 Horseradish peroxidase NA NA NA
G31568 β-Lactamase NA NA NA
A10533 Unlabeled NA NA NA
Goat Anti–Rabbit IgG, Highly Cross-Adsorbed
A21068 Alexa Fluor 350 Blue 346 442
A11034
R37116 †
Alexa Fluor 488 Green 495 519
O11038 Oregon Green 488 Green 496 524
A11035 Alexa Fluor 546 Orange 556 573
A21429 Alexa Fluor 555 Orange 555 565
A11036 Alexa Fluor 568 Red-orange 578 603
A11037
R37117 †
Alexa Fluor 594 Red 590 617
A21071 Alexa Fluor 633 Far-red 632 647
A31577 Alexa Fluor 635 Far-red 633 647
A21245 Alexa Fluor 647 Far-red 650 668
A21074 Alexa Fluor 660 Far-red 663 690
A21109 Alexa Fluor 680 Far-red 679 702
A11369 Alexa Fluor 790 Infrared 782 805
Goat Anti–Rabbit IgG, F(ab')2 Fragments
A11069 Alexa Fluor 350 Blue 346 442
A10526 Fluorescein Green 494 518
A11070 Alexa Fluor 488 Green 495 519
A11071 Alexa Fluor 546 Orange 556 573
A21430 Alexa Fluor 555 Orange 555 565
A10542 R-phycoerythrin (R-PE) Orange 496, 546, 565 578
A21069 Alexa Fluor 568 Red-orange 578 603
A11072 Alexa Fluor 594 Red 590 617
A21072 Alexa Fluor 633 Far-red 632 647
A21246 Alexa Fluor 647 Far-red 650 668
A21077 Alexa Fluor 680 Far-red 679 702
B21078 Biotin NA <300 NA
F21456 Alkaline phosphatase NA NA NA
A10547 Horseradish peroxidase NA NA NA
Chicken Anti–Rabbit IgG
A21441 Alexa Fluor 488 Green 495 519
A21442 Alexa Fluor 594 Red 590 617
A21443 Alexa Fluor 647 Far-red 650 668
Donkey Anti–Rabbit IgG
A10039 Alexa Fluor 350 Blue 346 442
A21206
R37118 †
Alexa Fluor 488 Green 495 519
A10040 Alexa Fluor 546 Orange 556 573
A31572 Alexa Fluor 555 Orange 555 565
A10042 Alexa Fluor 568 Red-orange 578 603
A21207
R37119 †
Alexa Fluor 594 Red 590 617
A31573 Alexa Fluor 647 Far-red 650 668
A10043 Alexa Fluor 680 Far-red 679 702
A11374 Alexa Fluor 790 Infrared 782 805
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. † ReadyProbes ready-to-use imaging reagent. NA = Not applicable.

Table 7.1C Molecular Probes affinity-purified anti–rat IgG antibody conjugates.

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Rat IgG
A21093 Alexa Fluor 350 Blue 346 442
A10528 Fluorescein Green 494 518
A11006 Alexa Fluor 488 Green 495 519
O6382 Oregon Green 488 Green 496 524
A10522 Cy3 Orange 552 570
A10531 Tetramethylrhodamine Orange 555 580
A11081 Alexa Fluor 546 Orange 556 573
A21434 Alexa Fluor 555 Orange 555 565
A10545 R-phycoerythrin (R-PE) Orange 496, 546, 565 578
A11077 Alexa Fluor 568 Red-orange 578 603
A11007 Alexa Fluor 594 Red 590 617
T6392 Texas Red-X Red 595 615
A21094 Alexa Fluor 633 Far-red 632 647
A10525 Cy5 Far-red 649 670
A10540 Allophycocyanin Far-red 650 660
A21247 Alexa Fluor 647 Far-red 650 668
A21096 Alexa Fluor 680 Far-red 679 702
A10517 Biotin NA <300 NA
D20697 DSB-X biotin NA <300 NA
A10546 Alkaline phosphatase NA NA NA
A10549 Horseradish peroxidase NA NA NA
A10536 Unlabeled NA NA NA
Goat Anti–Rat IgG, F(ab')2 Fragments
A10527 Fluorescein Green 494 518
A10544 R-phycoerythrin (R-PE) Orange 496, 546, 565 578
A10691 Cy5–R-PE Far-red 496, 546, 565 670
A10548 Horseradish peroxidase NA NA NA
Rabbit Anti–Rat IgG
A21210 Alexa Fluor 488 Green 495 519
A21211 Alexa Fluor 594 Red 590 617
Chicken Anti–Rat IgG
A21470 Alexa Fluor 488 Green 495 519
A21471 Alexa Fluor 594 Red 590 617
A21472 Alexa Fluor 647 Far-red 650 668
Donkey Anti–Rat IgG
A21208 Alexa Fluor 488 Green 495 519
A21209 Alexa Fluor 594 Red 590 617
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1D Molecular Probes affinity-purified anti–human IgG antibody conjugates.

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Human IgG
A11013 Alexa Fluor 488 Green 495 519
A21089 Alexa Fluor 546 Orange 556 573
A21433 Alexa Fluor 555 Orange 555 565
A21090 Alexa Fluor 568 Red-orange 578 603
A11014 Alexa Fluor 594 Red 590 617
A21091 Alexa Fluor 633 Far-red 632 647
A21445 Alexa Fluor 647 Far-red 650 668
Mouse Anti–Human IgG1
A10631 Alexa Fluor 488 Green 495 519
A10650 Biotin NA <300 NA
A10648 Horseradish peroxidase NA NA NA
A10630 Unlabeled NA NA NA
Mouse Anti–Human IgG4
A10663 Biotin NA <300 NA
A10654 Horseradish peroxidase NA NA NA
A10651 Unlabeled NA NA NA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1E Molecular Probes affinity-purified anti–sheep IgG antibody conjugates.

Cat. No. Label Emission Color Abs * Em *
Donkey Anti–Sheep IgG
A21097 Alexa Fluor 350 Blue 346 442
A11015 Alexa Fluor 488 Green 495 519
A21098 Alexa Fluor 546 Orange 556 573
A21436 Alexa Fluor 555 Orange 555 565
A21099 Alexa Fluor 568 Red-orange 578 603
A11016 Alexa Fluor 594 Red 590 617
A21100 Alexa Fluor 633 Far-red 632 647
A21448 Alexa Fluor 647 Far-red 650 668
A21102 Alexa Fluor 680 Far-red 679 702
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm.

Table 7.1F Molecular Probes affinity-purified anti–goat IgG antibody conjugates.

Cat. No. Label Emission Color Abs * Em *
Rabbit Anti–Goat IgG
A10529 Fluorescein Green 494 518
A11078 Alexa Fluor 488 Green 495 519
A10532 Tetramethylrhodamine Orange 555 580
A21085 Alexa Fluor 546 Orange 556 573
A21431 Alexa Fluor 555 Orange 555 565
A11079 Alexa Fluor 568 Red-orange 578 603
A11080 Alexa Fluor 594 Red 590 617
A21086 Alexa Fluor 633 Far-red 632 647
A21446 Alexa Fluor 647 Far-red 650 668
A21088 Alexa Fluor 680 Far-red 679 702
A10518 Biotin NA <300 NA
R21458 Alkaline phosphatase NA NA NA
R21459 Horseradish peroxidase NA NA NA
A10537 Unlabeled NA NA NA
Rabbit Anti–Goat IgG, F(ab')2 Fragments
A21222 Alexa Fluor 488 Green 495 519
A21223 Alexa Fluor 594 Red 590 617
Chicken Anti–Goat IgG
A21467 Alexa Fluor 488 Green 495 519
A21468 Alexa Fluor 594 Red 590 617
A21469 Alexa Fluor 647 Far-red 650 668
Donkey Anti–Goat IgG
A21081 Alexa Fluor 350 Blue 346 442
A11055 Alexa Fluor 488 Green 495 519
A11056 Alexa Fluor 546 Orange 556 573
A21432 Alexa Fluor 555 Orange 555 565
A11057 Alexa Fluor 568 Red-orange 578 603
A11058 Alexa Fluor 594 Red 590 617
A21082 Alexa Fluor 633 Far-red 632 647
A21447 Alexa Fluor 647 Far-red 650 668
A21083 Alexa Fluor 660 Far-red 663 690
A21084 Alexa Fluor 680 Far-red 679 702
A11370 Alexa Fluor 790 Infrared 782 805
D20698 DSB-X biotin NA <300 NA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1G Molecular Probes affinity-purified anti–chicken IgG antibody conjugates.

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Chicken IgG
A11039 Alexa Fluor 488 Green 495 519
A11040 Alexa Fluor 546 Orange 556 573
A21437 Alexa Fluor 555 Orange 555 565
A11041 Alexa Fluor 568 Red-orange 578 603
A11042 Alexa Fluor 594 Red 590 617
A21103 Alexa Fluor 633 Far-red 632 647
A21449 Alexa Fluor 647 Far-red 650 668
D20701 DSB-X biotin NA <300 NA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1H Molecular Probes affinity-purified anti–guinea pig IgG antibody conjugates.

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Guinea Pig IgG
A11073 Alexa Fluor 488 Green 495 519
A11074 Alexa Fluor 546 Orange 556 573
A21435 Alexa Fluor 555 Orange 555 565
A11075 Alexa Fluor 568 Red-orange 578 603
A11076 Alexa Fluor 594 Red 590 617
A21105 Alexa Fluor 633 Far-red 632 647
A21450 Alexa Fluor 647 Far-red 650 668
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates in nm.

Table 7.1I Molecular Probes affinity-purified goat anti–hamster IgG antibody conjugates.

Cat. No. Label Emission Color Abs * Em *
Goat Anti–Hamster IgG
A21110 Alexa Fluor 488 Green 495 519
A21111 Alexa Fluor 546 Orange 556 573
A21112 Alexa Fluor 568 Red-orange 578 603
A21113 Alexa Fluor 594 Red 590 617
A21451 Alexa Fluor 647 Far-red 650 668
* Approximate absorption (Abs) and emission (Em) maxima of conjugates, in nm.

Table 7.1J Molecular Probes affinity-purified goat anti–IgM (µ chain) antibody conjugates.

Fluorophore Abs/Em * Anti–Mouse IgM † Anti–Rat IgM † Anti–Human IgM †
Alexa Fluor 350 346/442 A31552    
Alexa Fluor 488 495/519 A21042 A21212 A21215
Alexa Fluor 546 556/573 A21045    
Alexa Fluor 555 555/565 A21426    
R-phycoerythrin 496, 546, 565/578 A10689
A10690 ‡
   
Alexa Fluor 568 578/603 A21043    
Alexa Fluor 594 590/617 A21044 A21213 A21216
Alexa Fluor 633 632/647 A21046    
Allophycocyanin 650/660 A10688    
Alexa Fluor 647 650/668 A21238 A21248 A21249
Alexa Fluor 680 679/702 A21048    
DSB-X biotin NA D20693    
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. † May also react with IgM from other species. ‡ F(ab')2 fragments. NA = Not applicable.
For Research Use Only. Not for use in diagnostic procedures.