Table 9.2 SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains.

Dye (Cat #) Ex/Em * Major Applications Features
SYPRO Ruby protein gel stain (S12000, S12001, S21900)280, 450/6102D gels, IEF gels, 1D SDS-PAGE
  • High sensitivity (1–2 ng/band; comparable to silver staining)
  • Linear quantitation range over three orders of magnitude
SYPRO Orange protein gel stain (S6650, S6651)300, 470/5701D SDS-PAGE
  • Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining)
  • Little protein-to-protein variability
  • Linear quantitation range over three orders of magnitude
SYPRO Red protein gel stain (S6653, S6654)300, 550/6301D SDS-PAGE
  • Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining)
  • Little protein-to-protein variability
  • Linear quantitation range over three orders of magnitude
SYPRO Tangerine protein gel stain (S12010)300, 490/6401D SDS-PAGE, blotting applications, zymography, electroelution
  • Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining)
  • Little protein-to-protein variability
  • Linear quantitation range over three orders of magnitude
  • No fixation required
Coomassie Fluor Orange protein gel stain (C33250, C33251)300, 470/5701D SDS-PAGE
  • Premixed and ready-to-use solution
  • Very good sensitivity (8 ng/band; higher than Coomassie brilliant blue staining)
  • Little protein-to-protein variability
  • Linear quantitation range over at least two orders of magnitude
All SYPRO gel and blot stains are compatible with Edman sequencing and mass spectrometry. * Excitation (Ex) and emission (Em) maxima, in nm. For maximum sensitivity, use excitation sources and optical filters matched to these values.
For Research Use Only. Not for use in diagnostic procedures.