Table 20.2 Summary of the pH response of our LysoSensor probes.

Cat. No.
LysoSensor Probe Abs/Em * (nm) pKa † Useful pH Range †
L7533 LysoSensor Blue DND-167 373/425 5.1 4.5–6.0
L7534 LysoSensor Green DND-153 442/505 7.5 6.5–8.0
L7535 LysoSensor Green DND-189 443/505 5.2 4.5–6.0
L7545 LysoSensor Yellow/Blue DND-160 384/540 ‡
329/440 §
4.2 3.5–6.0
* Absorption (Abs) and fluorescence emission (Em) maxima, at pH 5; values may vary somewhat in cellular environments. † All pKa values were determined in vitro; values are likely to be different in cells. ‡ At pH 3. § At pH 7.
For Research Use Only. Not for use in diagnostic procedures.