In solutions that contain several chelating species, or that contain ions such as Zn2+ that compete with Ca2+for binding to BAPTA or EGTA, calculating the free Ca2+ concentration requires simultaneously solving multiple equilibrium expressions. Except for the simplest cases where analytical solutions are feasible, researchers must use an iterative computer program to complete these calculations. MAXCHELATOR, a series of programs for determining free metal concentrations in the presence of chelators,ref is freely accessible on the Web.

Access the MAXCHELATOR series of computer programs

For Research Use Only. Not for use in diagnostic procedures.