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Pathway Focus: The Interferon Pathway

Cell Staining Simulation tool

Primary Antibody Search Tool

3D Cell and Cell Staining Simulation tool iPad app

Gibco® Recombinant Interferons

  • High biological activity
  • High purity
  • Proven compatibility with Gibco® media


Gibco® recombinant interferons are used in a wide variety of investigations requiring pathway stimulation. Gibco® recombinant interferons have been bioassayed in cells cultured using Gibco® media. So if you’re already using Gibco® media, you can have more confidence in the compatibility of these reagents in your cell culture–based assays.

Interferons are released in response to pathogens to trigger the immune system response. Upon binding to surface receptors, interferons activate the JAK/STAT pathway.

Many scientists use Gibco® recombinant interferons to stimulate cells during investigations of downstream signaling events. Gibco® recombinant interferons may also be used as standards in your assays.

We offer a range of human recombinant interferons—IFN-α, β, and γ. We also offer IFN-γ from human, mouse, rat, and swine.

Gibco® cytokines and growth factors are high-purity recombinant proteins with high bioactivity. To help ensure Gibco® recombinant proteins are of the highest quality, each protein is analyzed for purity and structural homogeneity.

Human INF-γ bioactivity assay
Figure 1. Human INF-γ bioactivity assay. The data above show the dose-dependent cytostasis of human WiDr cells. The ED50 for this lot is 0.761.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Recombinant Interferon Selection Guide

IFN-α Products
Size
 Cat. No.
IFN-α A Subtype Recombinant Human
106 units
PHC4814
IFN-α A Pure Recombinant Human
5 µg
PHC4014
IFN-α A/D Recombinant Human (liquid form)
105 units
PHC4044
IFN-α A/D Recombinant Human (liquid form)
1 × 105 units
PHC4064
IFN-α D Subtype Recombinant Human
106 units
PHC4054
IFN-α A Pure Recombinant Mouse
105 units
PMC4016
IFN-α Recombinant Rat
105 units
PRC4014


More Products Listed Below

IFN-β Products
Size
 Cat. No.
IFN-β1A Mammalian Recombinant Human
1 × 105 units
PHC4244
IFN-β Recombinant Rat
105 units
PRC4024


IFN-γ Products
Size
 Cat. No.
IFN-γ Recombinant Human
100 µg
PHC4031
IFN-γ Recombinant Human
1 mg
PHC4033
IFN-γ Recombinant Mouse
15 µg
PMC4034
IFN-γ Recombinant Mouse
100 µg
PMC4031
IFN-γ Recombinant Mouse
1 mg
PMC4033
IFN-γ Recombinant Rat
10 µg
PRC4034
IFN-α Recombinant Rat
100 µg
PRC4035
IFN-γ Recombinant Rat
1 mg
PRC4033
IFN-γ Recombinant Swine
10 µg
PSC4034
IFN-γ Recombinant Swine
100 µg
PSC4031

ABfinity™ Recombinant Monoclonal Antibodies—Identify Downstream Interferon Signaling Events

  • Validated and extensively characterized
  • Deliver consistent performance for your assays
  • Reliable sensitivity and specificity


ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. ABfinity™ antibodies are available for many targets, including JAK1 [phospho-Y1022], STAT1 [phospho-Y701], STAT4, and STAT6 [phospho-Y641].

JAK/STAT signaling is important in regulating immune responses and autoimmune diseases, especially when interferon-γ signaling is compromised in various viral infections. ABfinity™ recombinant monoclonal antibodies are ideal reagents for signaling pathway research. ABfinity™ technology helps ensure consistent antibody performance, so you don’t have to reoptimize your assay with each new lot.

Phosphorylated STAT1 (Y701) detected in interferon-γ–stimulated or unstimulated HeLa cells
Figure 2. Phosphorylated STAT1 (Y701) detected in interferon-γ–stimulated or unstimulated HeLa cells.

HeLa cells were stimulated with (top right) or without (top left) 100 ng/mL interferon-γ and labeled with STAT1 [pY701] ABfinity™ Recombinant Rabbit Monoclonal Antibody (1 µg/mL). Stimulated cells were preincubated with phosphopeptide used as an immunogen (bottom right) or with nonphosphopeptide (bottom left), demonstrating phosphospecificity. Alexa Fluor® 488 goat anti–rabbit IgG at 1:1000 dilution was used as secondary antibody.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Ordering information

Product
Species
Quantity
Cat. No.
STAT1 [pY701] ABfinity™ Recombinant Rabbit Monoclonal Antibody – Purified 
Human
100 μg
700349
STAT4 - ABfinity™ Recombinant Rabbit Monoclonal Antibody – Purified
Human
100 μg
700185
STAT6 [pY641] ABfinity™ Recombinant Rabbit Monoclonal Antibody – Purified
Human
100 μg
700247
JAK1 [pY1022/pY1023] ABfinity™ Recombinant Rabbit Monoclonal Antibody – Purified
Human
100 μg
700028

Rapidly and Reliably Measure Secreted Interferon

  • Measure interferons (IFN) reliably using ELISA
  • Measurements based on standards for improved accuracy
  • Kits for multiple IFN types and species are available


Interferons are perhaps best known for their immunomodulatory properties—activating immune system cells such as macrophages and natural killer cells.

 

In addition, interferons are secreted in the bloodstream in response to invaders such as viruses and bacteria, and are thought to play a role in regulating cellular growth and fighting certain tumors. Different types of interferons such as IFN-β, -α, and - utilize different cell-surface receptors.

Invitrogen™ ELISA kits allow you to easily quantify interferons secreted in blood (including serum and plasma) or tissue culture supernatants. 

The sandwich ELISA technique is the gold standard for cytokine measurement, giving you more confidence in the accuracy of the results you obtain.

  • Learn More About Invitrogen™ ELISA Kits
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Interferon ELISA Kit Selection Guide

Product
Species
Quantity
Cat. No.
IFN-β Human ELISA Kit
Human
96 tests
KAC1201
IFN-α (multi-subtypes) Human ELISA Kit
Human
96 tests
KHC4032
IFN-γ Human ELISA Kit
Human
96 tests
192 tests
480 tests
KHC4021
KHC4022
KHC4021C
IFN-β Mouse ELISA Kit
Mouse
96 tests
KMC4041


More Products Listed Below 

Product
Species
Quantity
Cat. No.
IFN-α (multi-subtype) Mouse ELISA Kit
Mouse
96 tests
KMC4011
IFN-γ Rat ELISA Kit
Rat
96 tests
192 tests
KRC4021
KRC4022
IFN-γ Swine ELISA Kit
Swine
96 tests
192 tests
KSC4021
KSC4022
IFN-γ Monkey ELISA Kit
Monkey
96 tests
192 tests
KPC4021
KPC4022
IFN-γ Bovine ELISA Kit
Bovine
96 tests
KBC1231