BioProbes® 69—Journal of Cell Biology Applications

 

FEATURED ARTICLE
Automated imaging. Simplified.
Here we describe the EVOS® FL Auto Imaging System. With automated stage movement, filter and objective changes, focus, and exposure, this multi-channel fluorescence imaging system gives you access to advanced applications such as time-lapse imaging, multiwell plate scanning, and image stitching and tiling. When equipped with the optional onstage incubator, the EVOS® FL Auto system allows you to acquire high-resolution time-lapse movies spanning many hours. Novice users are able to navigate the intuitive touch screen controls, and experienced users will appreciate the superb quality of the images produced.
Read more

 

No better time to react: Painting biomolecules with reactive fluorophores

The poster in the center of BioProbes® 69 describes our extensive selection of reactive fluorophores for labeling antibodies, other proteins and peptides, small molecules, and oligonucleotides, along with some key examples of their use in FISH, immunocytochemistry, and flow cytometry. Hang this poster on your lab fridge, and your labeling options will be right where you need them.

Download the poster (1.2 MB)

  Molecular Probes® fluorophore poster  

Featured new products

Just released
Our newest cell and molecular biology products and technologies

Cell imaging

Automated imaging. Simplified.
The EVOS® FL Auto Imaging System

Convenient dropper bottles mean no calculations or dilutions required: Simply add and image
ReadyProbe® ready-to-use imaging reagents for brilliant results

Flow cytometry

Checking vital signs: Don’t let dead cells mislead you
Molecular Probes® cell health assays for flow cytometry

Expand your multicolor capacity with the Pacific Green™ dye
New violet laser–excitable Pacific Green™ dye and conjugates for flow cytometry

Protein expression and localization

Multiplex western blotting with fluorescent antibody probes
Long-wavelength Qdot® and Alexa Fluor® conjugates for sensitive and quantitative detection

A new paradigm for site-selective labeling of antibodies
The SiteClick™ antibody labeling system

Cell signaling

Oxidative stress from a cell’s point of view
From CellROX® ROS sensors to Image-iT® and Click-iT® lipid peroxidation detection kits

Autophagy made visible
Advanced fluorescent probes for imaging distinct processes in the autophagy pathway

Live-cell staining of pluripotent stem cells
Alkaline Phosphatase Live Stain for early identification of induced pluripotent stem cells

Target key proteins in cell signaling pathways
ABfinity™ recombinant antibodies for cancer research

Journal club

A new application for pHrodo™ dextrans: Visualizing individual synaptic vesicles

 

Online and on the move

 

The SpectraViewer tool takes a broader view

We are pleased to announce the release of the new and improved SpectraViewer tool. The updated SpectraViewer tool allows you to plot up to 14 fluorophores on a single graph, which you can then print, save, or export as a .txt file. Once you have selected fluorophores, you can easily generate a product list using the “Review Products and Purchase” button.

On each SpectraViewer graph, you can also designate as many as 7 laser lines and 10 or more excitation and emission filters. Furthermore, you can load preconfigured instruments, including lasers and filters, and save these specifications for later use. A spillover table option lets you view calculated overlaps with other spectra and filters. And unlike the original version, which only offered spectral data for Molecular Probes® fluorophores, the new SpectraViewer tool will continually be updated with as many spectra, instruments, and filters as possible.

 

Custom GeneArt® services: From gene to purified protein in 30 business days

The GeneArt® Genes-to-Protein Service provides you with an efficient and affordable option for outsourcing your protein expression and purification work. We employ the Expi293™, FreeStyle™ 293, and CHO Expression Systems to rapidly produce proteins through transient expression in mammalian cells. These chemically defined systems use mammalian cells specially adapted for high-density, serum-free suspension culture. Mammalian expression systems facilitate proper protein folding and posttranslational modifications that may not occur in nonmammalian systems. We recommend you order an expression-optimized gene and let us clone it into a Life Technologies expression vector, though we can also use your expression plasmid. The combination of gene expression optimization and advanced expression systems routinely leads to a high overall success rate and excellent protein yields.

To get started, download the Protein Production Request Form at from the GeneArt® genes to proteins web page and email the completed form to geneartprotein@lifetech.com. Dedicated project managers will analyze the project according to your requirements and provide a quote and timeline within 2–3 business days. Depending on the gene length, the entire workflow from gene synthesis to purified protein is typically completed within 30 business days.

 

Cell health: What do you want to detect?

Molecular Probes® reagents and assay kits let you validate your processes and help ensure that your experiment is on track. With the right probes, you can see how your cells are responding to changing conditions, follow key indicators of cell vitality, and ask the critical questions that move you on to the next experimental step.

Our online cell health tools help you select the right approach for your questions. Are my cells alive and proliferating? Are they performing basic functions? Are they healthy or stressed? To find the answer to each question, you are introduced to a choice of platforms, techniques, and approaches so that you can choose what works best in your experiment and in your lab. All of the options are presented with an integral bibliography so that you can browse publications and compare results as you go. Online guides are available for the following application areas:

  • Cell viability
  • Cell proliferation
  • Cell cycle
  • Cell tracking and tracing
  • Cytotoxicity
  • Endocytosis/phagocytosis
  • Oxidative stress and damage
  • Apoptosis
  • Autophagy
  • Mitochondrial function
  • Ion indicators
  • Immunofluorescence

See our cell health tools and product selection guides

 

Exosome research: New strategies and new products

Now that exosomes have been identified in large numbers in all body fluids, the field of exosome research is exploding with new strategies for analyzing their composition, functions, networks, and pathways. The Life Technologies website contains a new area devoted to exosome research. Take a look at our exosome portal to find products for isolation, characterization, and analysis; links to instructional videos and webinars; and more.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.