BioProbes® 70—Journal of Cell Biology Applications
Our research collaboration with The Parkinson’s Institute (Sunnyvale, California) has set about to develop Parkinson's disease model systems using neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSCs) that were generated from donor fibroblasts. Here we describe two of the assays we used to evaluate NSC identity and health.
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We are bringing our award-winning BioProbes® articles to you even sooner. We will be publishing new BioProbes® articles online every month to keep you up-to-date on new fluorescence technologies and cell biology applications. Check back frequently and watch BioProbes® 70 take shape!
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Evaluate neural stem cells for neurodegenerative disease models
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The Molecular Probes® flow cytometry app—This. Is. So. Easy.
The Molecular Probes® Flow Cytometry Reagent Guide and Protocols mobile app is designed to help you find fluorescent reagents, kits, and protocols for cell biology–related flow cytometry applications. Features include product guides for instrument calibration and setup. detailed examples of data output, and excitation and emission data for every product.
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Expert opinion: The basics of multicolor flow cytometry panel design
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters, multicolor flow cytometry has become more popular and more accessible than ever. When designing polychromatic flow cytometry panels, there are dozens of factors to consider. As the complexity of the experiment increases, so does the number of parameters that need to be analyzed and the technical issues that need to be addressed.
To help sort through the challenges of panel design, we reached out to an expert in flow cytometry: Holden Maecker, PhD. Dr. Maecker is the Director of the Human Immune Monitoring Center and also an Associate Professor of Microbiology and Immunology at Stanford University. In the “Basics of Multicolor Flow Cytometry Panel Design” webinar, Dr. Maecker covers the key rules for choosing fluorescent dye combinations and filter sets and illustrates these rules with plenty of examples and practical applications. He also discusses controls and standardization, which are absolutely critical in ensuring quality data from multicolor flow panels, as well as the relevance of panel design to new mass cytometry platforms.