BioProbes 70  

FEATURED ARTICLE
Evaluate neural stem cells for neurodegenerative disease models—
Neural stem cell–specific protein expression and mitochondrial assessment

Our research collaboration with The Parkinson’s Institute (Sunnyvale, California) has set about to develop Parkinson's disease model systems using neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSCs) that were generated from donor fibroblasts. Here we describe two of the assays we used to evaluate NSC identity and health.

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Evaluate neural stem cells for neurodegenerative disease models
Neural stem cell–specific protein expression and mitochondrial assessment

Journal Club

An In-Depth Look at Fluorescent Dyes for Organelle Labeling

Online and on the move

Molecular Probes® cell imaging app  

Essential imaging tools in one place:
The Molecular Probes® cell imaging app

The Molecular Probes® Cell Imaging Reagent Guide and Protocols mobile app is designed to help you find fluorescent reagents, kits, and protocols for cell biology–related fluorescence microscopy applications. Also included are product guides for instrument calibration and setup, protein labeling, and secondary detection, as well as examples of data output. Two key features include: (1) a built-in protocol timer that runs even if you leave the app and (2) excitation and emission data for every product.

Download the Molecular Probes® Cell Imaging Reagent Guide and Protocols mobile app today:

Molecular Probes® flow cytometry app  

The Molecular Probes® flow cytometry app—This. Is. So. Easy.

The Molecular Probes® Flow Cytometry Reagent Guide and Protocols mobile app is designed to help you find fluorescent reagents, kits, and protocols for cell biology–related flow cytometry applications. Features include product guides for instrument calibration and setup. detailed examples of data output, and excitation and emission data for every product.

Download the Molecular Probes® Flow Cytometry Reagent Guide and Protocols mobile app today:

The basics of multicolor flow cytometry panel design  

Expert opinion: The basics of multicolor flow cytometry panel design

With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters, multicolor flow cytometry has become more popular and more accessible than ever. When designing polychromatic flow cytometry panels, there are dozens of factors to consider. As the complexity of the experiment increases, so does the number of parameters that need to be analyzed and the technical issues that need to be addressed.

To help sort through the challenges of panel design, we reached out to an expert in flow cytometry: Holden Maecker, PhD. Dr. Maecker is the Director of the Human Immune Monitoring Center and also an Associate Professor of Microbiology and Immunology at Stanford University. In the “Basics of Multicolor Flow Cytometry Panel Design” webinar, Dr. Maecker covers the key rules for choosing fluorescent dye combinations and filter sets and illustrates these rules with plenty of examples and practical applications. He also discusses controls and standardization, which are absolutely critical in ensuring quality data from multicolor flow panels, as well as the relevance of panel design to new mass cytometry platforms.

For Research Use Only. Not for use in diagnostic procedures.