Fluorescent proteins are highly versatile biological markers for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. To complement these powerful tools, we offer anti-GFP and anti-RFP antibodies and antibody conjugates for a wide variety of applications, including imaging, western blotting, and flow cytometry. All six of our anti-GFP antibodies are suited for detection of native GFP, GFP variants, and most GFP fusion proteins. The affinity-purified anti-RFP antibody is used to detect native TagRFP and most fusion proteins derived from Entacmaea quadricolor.

Sensitive and Specific Detection

Anti-GFP and anti-RFP antibodies provide a convenient method for visualizing GFP and RFP, especially when amplification of the fluorescent protein of interest is necessary to overcome a dim or degraded signal. Our anti-GFP and anti-RFP antibodies are easily incorporated into standard immunostaining protocols for cell and tissue analysis (Figure 1).

Invitrogen™ anti–fluorescent protein antibodies are also consistent and reliable tools for detecting fluorescent proteins and fluorescent protein fusions in western blot and flow cytometry applications. These antibodies are highly specific—they only react with the intended target—which contributes to low background signal and increases signal to noise.

Validated for a Range of Applications

Invitrogen™ antibodies have been validated for a broad range of applications and targets. Find antibodies for your application.

Immunolabeling enhances GFP fluorescence
Figure 1. Immunolabeling enhances GFP fluorescence. HeLa cells were transduced with CellLight® Lysosomes-GFP, then fixed and permeabilized. GFP, localized in the lysosomes, was labeled with anti-GFP rabbit monoclonal antibody and detected with Alexa Fluor® 594 goat anti–rabbit IgG (H+L) (red). The nucleus was stained with DAPI (blue), and the sample was mounted using ProLong® Gold antifade reagent. (Left panel) Some GFP fluorescence was retained in the lysosomes after fixation. (Center panel) Immunolabeling and detection greatly improved visualization by enhancing the dim GFP fluorescence. (Right panel) The merged yellow signal indicates colocalization of GFP fluorescence and the detection antibody.

Ordering Information

Sku Name Size Price Qty
A6455 GFP Rabbit Serum Polyclonal Antibody 100 µL USD 207.00
A10262 GFP Chicken IgY Antibody Fraction 100 µL USD 297.00
A11122 GFP Rabbit IgG Polyclonal Antibody Fraction 100 µL USD 310.00
A11120 GFP (clone 3E6) Mouse IgG2a, Monoclonal Antibody 100 µg USD 246.00
A11121 GFP (clone 11E5) Mouse IgG1, Monoclonal Antibody 100 µg USD 246.00
G10362 GFP Monoclonal Antibody, ABfinity™ Recombinant 100 µg USD 260.00
R10367 Red Fluorescent Protein (RFP) Rabbit Polyclonal Antibody, Purified 100 µg USD 263.00
A21311 GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 488 Conjugate 100 µL USD 310.00
A31851 GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 555 Conjugate 100 µL USD 316.00
A21312 GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 594 Conjugate 100 µL USD 310.00
A31852 GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 647 Conjugate 100 µL USD 207.00
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.