When Inflammatory Cytokines Are Unbalanced

Investigate the Role of Inflammation in Disease States Using Immunoassays

(See a complete list of products discussed in this article.)


As part of the immune response, inflammation plays an important role in defending the body against pathogens such as viruses, bacteria, fungi, and other parasites. However, inappropriate activation of inflammatory processes is an underlying contributor to many common pathological conditions. For example, autoimmune diseases arise when our immune system mistakes our cells or tissues for pathogens and attacks them. In addition, tumor proliferation and metastasis may occur when inflammatory cytokines create a microenvironment conducive to cancer progression. Figure 1 shows a simplified representation of cytokine interactions during inflammation.

Simplified representation of cytokine interactions during inflammation
Figure 1. Simplified representation of cytokine interactions during inflammation.

Acute Versus Chronic Inflammation

Acute inflammation is a short-lived response that is characterized by extravasation of leukocytes, erythrocytes, and plasma components into the injured tissue. Left unchecked, the acute inflammatory process can lead to chronic inflammation.

Unlike acute inflammation, chronic inflammation is characterized primarily by tissue infiltration by lymphocytes and macrophages. Chronic inflammation is closely associated with allergy, atherosclerosis, cancer, arthritis, and Alzheimer’s disease, as well as autoimmune diseases. The process of acute inflammation is well defined, but the causes of chronic inflammation and its associated molecular and cellular pathways are still not well understood [1].

The Critical Balance Between Pro- and Anti‑inflammatory Mediators

The overall effect of an inflammatory response is dictated by the balance between pro- and anti-inflammatory mediators (Figure 2). Pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α are responsible for early responses and amplify inflammatory reactions, whereas anti-inflammatory cytokines, which include IL-4, IL-10, and IL-13, have the opposite effect in that they limit the inflammatory responses (Table 1). The increasing complexity of pro- and anti-inflammatory cytokine/chemokine networks has made it crucial to examine them in relevant functional groups rather than individually (Figure 1).

To facilitate the investigation of these pro- and anti-inflammatory cytokines, Life Technologies has developed a large portfolio of single and multiplex assay kits designed to profile and quantitate levels of secreted cytokine proteins in body fluids such as plasma and serum, as well as in in vitro culture supernatants.

 

Inflammation is required for a healthy immune response, but it is also responsible for the pathogenesis of several diseases 
Figure 2. Inflammation is required for a healthy immune response, but it is also responsible for the pathogenesis of several diseases. The balance between pro- and anti-inflammatory cytokines plays a critical role in the body’s response to an inflammatory stimulus.

 

Table 1. Pro- and anti-inflammatory mediators. From Bone et al. [2].

Pro-inflammatory mediators

  • Cell-surface CD14
  • Free radical generation
  • IL-1β
  • IL-2
  • IL-6
  • IL-8
  • IL-15
  • IFN-γ
  • Leukemia inhibitory factor
  • Monocyte chemoattractant protein-1
  • Monocyte chemoattractant protein-2
  • Neopterin
  • Neutrophil elastase
  • Phospholipase A2
  • Plasminogen activator inhibitor-1
  • Platelet activating factor
  • Prostacyclins
  • Prostaglandins
  • Protein kinase
  • Soluble adhesion molecules
  • Thromboxane
  • TNF-α
  • Tyrosine kinase
  • Vasoactive neuropeptides

Anti-inflammatory mediators

  • Epinephrine
  • IL-4
  • IL-10
  • IL-13
  • IL-1RA
  • Leukotriene B4-receptor antagonism
  • LPS binding protein
  • Soluble recombinant CD-14
  • Soluble TNF-α receptors
  • Transforming growth factor-β
  • Type II IL-1R

Multiplex Cytokine Assay Panels for the Luminex® Platform

The Novex® multiplex cytokine assay panels enable the accurate, reproducible, and sensitive profiling of multiple secreted cytokines, chemokines, and growth factors simultaneously in a single well using the Luminex® platform. Available for analysis of human, monkey, mouse, and rat proteins, these assay panels provide more data from each sample of plasma, serum, or tissue culture supernatant than do ELISAs, an important feature when sample size is limited.

Furthermore, the Novex® cytokine panels are available either with antibody-coated polystyrene capture beads or with the recently introduced antibody-coated magnetic capture beads, which facilitate automation, decrease hands-on time, and increase throughput and precision. We have used the Human Cytokine Magnetic 25-Plex Panel to analyze the cytokine and chemokine levels in seven different serum samples in a single experiment (Figure 3). Luminex® technology has become increasingly adopted for the savings it provides in time, cost, and sample volume, and it now has been cited in over 7,000 scientific publications. These Novex® assay panels are designed for use with the Luminex® 100⁄200™, FLEXMAP 3D®, and MAGPIX® systems.

 

Seven serum samples are analyzed in a single experiment using the Human Cytokine 25-Plex Magnetic Panel 
Figure 3. Seven serum samples are analyzed in a single experiment using the Human Cytokine Magnetic 25-Plex Panel. Serum samples from seven different individuals were assayed with the Novex® Human Cytokine Magnetic 25-Plex Panel to determine the levels of 25 different cytokines and chemokines simultaneously. Measurements were performed using the Luminex® 200™ system.

Singleplex Cytokine ELISA Kits

Novex® ELISA kits can be used for single-protein measurement to further evaluate individual cytokine targets. To supplement our colorimetric ELISA kits, we have added chemiluminescence-based ELISA kits for improved sensitivity and a broader dynamic range. These Novex® Chemi ELISA kits, which employ a chemiluminescent substrate as an indicator of antigen levels, undergo rigorous validation for sensitivity, specificity, precision, and lot-to-lot consistency.

The Whole Picture

Dissecting the complex process of inflammation requires a multiplex approach using tools that are designed to work together. Learn more about our Novex® immunoassay reagents and kits.

Ordering Information

Product Targets Quantity Cat. No.
Human Cytokine Magnetic 25-Plex PanelEotaxin, GM-CSF, IFN-α, IFN-γ, IL-1β, IL-1RA, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, IP-10, MCP‑1, MIG, MIP-1α, MIP-1β, RANTES, TNF-α100 testsLHC0009M
Monkey Cytokine Magnetic 28-Plex PanelEGF, eotaxin, FGF-basic, G-CSF, GM-CSF, HGF, IFN-γ, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-15, IL-17, I-TAC, MCP-1, MDC, MIF, MIG, MIP-1α, MIP-1β, RANTES, TNF-α, VEGF100 testsLPC0003M
Mouse Cytokine Magnetic 20-Plex PanelFGF basic, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p40/p70), IL-13, IL-17, IP-10, KC, MCP-1, MIG, MIP-1α, TNF-α, VEGF100 testsLMC0006M
Rat Cytokine 10-Plex PanelGM-CSF, IL-1α, IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12 (p40/p70), TNF-α100 testsLRC0002
Hu IL-1β Chemi ELISA KitHu IL-1β96 testsKHC0019
Hu IL-6 Chemi ELISA KitHu IL-696 testsKHC0069

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