New Click-iT® Lipid Peroxidation Imaging Kit

The Click-iT® Lipid Peroxidation Imaging Kit and stand-alone Click-iT® LAA reagent employ click chemistry and an alkyne-modified linoleic acid (linoleamide alkyne or LAA) to detect lipid peroxidation–derived protein modifications by fluorescence microscopy or high-content screening. When incubated with cells, Click-iT® LAA incorporates into cell membranes. Upon lipid peroxidation, LAA is oxidized and produces 9- and 13-hydroperoxy-octadecadienoic acid (HPODE). These hydroperoxides decompose to α,β-unsaturated aldehydes, which readily modify proteins at nucleophilic side chains, producing alkyne-containing proteins that can then be detected with fluorescent or biotinylated azides using click chemistry.

The Click-iT® Lipid Peroxidation Imaging Kit provides the tools needed to visualize the lipid peroxidation modifications using green-fluorescent Alexa Fluor® 488 azide. If you’d like to customize the click reaction, we also offer the stand-alone LAA reagent, as well as a variety of azide-modified detection reagents and the Click-iT® Cell Reaction Buffer Kit.

 

BioProbes Lipid peroxidation detection in hemin-treated cells. After incubation with 50 µM LAA in complete growth medium at 37°C for 30 min, bovine pulmonary artery endothelial (BPAE) cells were treated with 10 μM hemin to induce lipid peroxidation–derived protein modifications (right), or left untreated (left) for 2 hr at 37°C, then fixed and permeabilized. The click reaction was performed with 5 µM Alexa Fluor® 488 azide from the Click-iT® Lipid Peroxidation Kit for 30 min. Cells were stained with NucBlue® Live Cell Stain and imaged on a Zeiss® Axiovert inverted microscope using a 40x objective.

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Trial Sizes for CellLight® and Premo™ FUCCI Reagents

Our popular CellLight® and Premo™ FUCCI reagents use BacMam technology to provide highly efficient, transient fluorescent protein labeling of live cells, including neurons and stem cells. New small pack sizes make it easy to try these premier live-cell organelle and cell cycle markers with your cells.

CellLight® reagents combine the utility and selectivity of fluorescent proteins (CFP, GFP, and TagRFP) with the transduction efficiency of BacMam 2.0, enabling unambiguous staining of organelles and cellular structures in live mammalian cells. Premo™ FUCCI Cell Cycle Sensor is a fluorescent two-color sensor of cell cycle progression and division in live cells. Cells change from red in the G1 phase, to yellow in G1/S interphase, to green in the S, G2, and M phases. Both CellLight® and Premo™ FUCCI reagents are easy to use—simply add, incubate, and image.

 

BioProbes 
CellLight® reagents expressed in live cells.  (A) CellLight® Actin-RFP and CellLight® Tubulin-GFP in human neonatal epidermal keratinocytes (HEKn). (B) CellLight® Mitochondria-RFP and CellLight® Lysosomes-GFP in HeLa cells counterstained with Hoechst® 33342.

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SYBR® GreenER™ Dye Shines in Real-Time PCR Master Mix

SYBR® Select Master Mix is a superior, cost-effective solution for your real-time PCR applications, delivering highly specific and sensitive quantitation with reliable single-copy detection. SYBR® Select Master Mix offers significant improvements over other master mixes by employing AmpliTaq® DNA Polymerase UP (Ultra Pure), which is activated by a proprietary hot-start mechanism. SYBR® Select Master Mix is also formulated with SYBR® GreenER™ dye—which is less inhibitory to PCR than SYBR® Green I dye, resulting in brighter signals—as well as heat-labile uracil-DNA glycosylase (UDG) for worry-free carryover contamination control. We offer SYBR® Select Master Mix in six sizes, from a 1 mL tube (100 reactions) to a 50 mL bottle (5,000 reactions).

 

BioProbes 
Melt curves for 8 different amplification reactions (4 replicates each) using the SYBR® Select Master Mix. Single Tm peaks were obtained for all assays, regardless of amplicon length and GC content, demonstrating that all assays generated a single amplicon without primer-dimer or nonspecific amplification.

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Catalog # Name Size List Price (USD) Qty
4472903 SYBR® Select Master Mix 1 x 1 mL 73.00
4472908 SYBR® Select Master Mix 1 x 5 mL 327.00
4472918 SYBR® Select Master Mix 2 x 5 mL 628.00
4472919 SYBR® Select Master Mix 5 x 5 mL 1,458.00
4472920 SYBR® Select Master Mix 10 x 5 mL 2,770.00
4472913 SYBR® Select Master Mix 1 x 50 mL 2,755.00

New Murine CD Antibody Markers for Flow Cytometry

Life Technologies now offers you several choices of fluorescent CD45 allele–specific monoclonal antibodies, providing more options for your multiplex flow cytometry experiments. The protein tyrosine phosphatase CD45 is an essential regulator of leukocyte activation and development. CD45 exists in allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. Our new CD45 allele–specific antibodies can provide critical information when the resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.

 

BioProbes 
CD45.2 detection in BALB/c splenocytes by flow cytometry. Splenocytes were stained with 0.5 μg Mouse IgG2a,κ Isotype Control PerCP-Cy®5.5 (open histogram) or 0.5 μg Anti–Mouse CD45.2 PerCP-Cy®5.5 (filled histogram). Cells in the lymphocyte gate were used for this analysis.

The Largest Multiplex Monkey Cytokine Panel on the Market

The Monkey Cytokine Magnetic 29-Plex Panel for the Luminex® platform is the largest multiplex panel on the market specifically designed for quantifying monkey cytokines in serum, plasma, and tissue culture supernatants. By measuring 29 analytes simultaneously, the Luminex® assay panel provides more data from each sample, saving both money and time compared with more traditional detection assays such as ELISAs. The panel is suitable for use with the Luminex® 200™, FLEXMAP 3D®, and MAGPIX® systems.

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Catalog # Name Size List Price (USD) Qty
LPC0005M Cytokine Monkey Magnetic 29-Plex Panel 100 assays 3,370.00

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.