General Procedures and Protocols for Using CaptureSelect® Affinity Products

General affinity procedures

A detailed protocol is designed for the use of each CaptureSelect® affinity product. Each product group is designed to work with a range of standard applications. POROS® CaptureSelect® analytical columns are designed for rapid quantitation and small-scale sample preparation of immunoglobulins, fusion proteins, antibody fragments (Fabs), and bispecific antibodies. Antibody Toolbox® products are used for the purification of antibodies and antibody fragments from serum, plasma, and cell culture supernatants.

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Proteomics Toolbox® products are used to improve proteomics analysis by depleting human plasma of selected abundant proteins. Biotinylated CaptureSelect® ligands are used for the detection, quantitation, and characterization of specific antibodies in applications such as capture ELISA and other immunoassays, and in label-free detection platforms like bio-layer interferometry (BLI) and surface plasmon resonance (SPR). CaptureSelect® protein purification products are used for the detection and purification of proteins from recombinant sources.

CaptureSelect® Affinity Protocols

Here is a complete list of the current products, with general protocol links:

 

POROS® CaptureSelect® analytical column general protocol 
Antibody Toolbox® general protocol for antibody purification
Protein purification general protocol
Proteomics Toolbox® general protocol for sample preparation
Conjugated ligand general protocol

Abbreviations: Hu, human; Ig, immunoglobulin; LC, light chain; MS, multi-species; mur, murine; ung, ungulate.