Features of Novex® ELISA kits
  • Sensitive, accurate, and consistent performance
  • Validated on serum, plasma, cell culture supernatant, or cell lysate samples
  • Ready-to-use, convenient assay

 

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Comparison of sensitivity and range of different types of Novex
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Figure 1. Comparison of sensitivity and range of different types of Novex® ELISA kits: chemiluminescent, ultrasensitive colorimetric, and standard colorimetric.

 

Our ELISA kits help provide accurate and consistent results (Figure 1). We research each target protein and calibrate our ELISA kits to provide physiologically relevant sensitivity. In addition, kits are validated using common sample types including serum, plasma, and cell culture supernatant. Cell lysates are used to validate kits that detect signaling proteins or phosphorylation. Novex® ELISA kits must meet rigorous quality-control specifications.

Our ELISA kits are manufactured in an ISO 13485
facility with stringent quality controls to ensure excellent quality and reproducibility (Figures 2–9).

 

Figure 2. Novex® ELISA kits provide reproducible results from lot to lot. Individual production lots (A–K) of the Novex® IFN-γ Human ELISA Kit were analyzed using 4 concentrations of control target protein (C1–C4). All 11 lots provided uniform sensitivity and low lot-to-lot variation (<20%).

 

Figure 4. High specificity of the STAT3 [pY705] phosphoELISA™ kit: peptide competition. Peptide blocking is performed on each kit to confirm specificity of the phosphorylation site. The phosphorylated tyrosine 705 blocks the ELISA signal, but the nonphosphorylated peptide sequence and another phosphopeptide do not.

 

Figure 6. The expression of α-synuclein in various cell lines, detected by α-synuclein ELISA. The α-synuclein ELISA kit is specific for measuring α-synuclein and is consistent with western blotting (inset). The blot was probed with α-synuclein rabbit polyclonal antibody and developed using an alkaline phosphatase–conjugated anti–rabbit IgG followed by a chemiluminescent substrate and autoradiography.

 

Figure 8. Correlation between recombinant and natural human vascular endothelial growth factor (hVEGF). Natural hVEGF was serially diluted in Standard Diluent Buffer. The optical density of each dilution was plotted against the standard curve. The closely aligned values of the natural and recombinant proteins indicate that the standard accurately reflects natural human VEGF content in samples.

 

Figure 3. phosphoELISA™ Kit standards are comparable to natural samples. Recombinant standards are tested against cell lysates to ensure correct measurement  values of natural samples. Note that the standard is closely aligned to the natural sample used.

 

Figure 5. High specificity of the phosphoELISA™ kit: no cross-reactivity. HEL cell lysates were incubated with the capture antibody used in the STAT5a [pY694] ELISA (lane 2). An antibody specific for STAT5a and STAT5b was used as a positive control (lanes 1 and 4). IgG beads were used as a negative control (lane 3). The capture antibody recognizes the a isoform of STAT5 but not the b isoform. Thus, the STAT5a [pY694] ELISA does not cross-react with the STAT5b protein.

 

Figure 7. Correlation between recombinant and natural Hu Tau. Natural human tau protein was serially diluted in Standard Diluent Buffer. The optical density of each dilution was plotted against the standard curve obtained using the Tau (Total) Human ELISA Kit. The closely aligned values of the natural and recombinant proteins
indicate that the standard accurately reflects natural human tau content in samples.

 

Figure 9. Representative standard curves for human IL-8 ELISA kits. Standard curves generated using the Novex® IL-8 Human ELISA Kit (normal) and the Novex® IL-8 Human Ultrasensitive ELISA Kit (ultrasensitive).