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Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5' phosphateterminated oligoribonucleotides and single-stranded DNA. It is suitable for removing mRNA during second strand cDNA synthesis, removing the poly(A) sequences from mRNA in the presence of oligo(dT), and for oligodeoxyribonucleotide-directed cleavage of RNA. RNase H is purified from E. coli expressing the E. coli RNase H gene on a plasmid.

Unit Definition

One unit is defined as the amount of RNase H that solubilizes 1 nmol poly(A) in 20 minutes at +37°C

Storage Buffer

20 mM Tris-HCl (pH 7.5)
100 mM KCl
10 mM MgCl2
0.1 mM EDTA
0.1 mM DTT
50 μg/ml BSA
50% (v/v) glycerol

Quality Control

Product qualification is described in the Certificate of Analysis (CofA), available on our website by product lot number at

Ordering Information

Sku Name Size Price Qty
18021071 Ribonuclease H 120 units USD 419.00
18021071.pps       25-May-2007