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It's about time old electrophoresis myths were busted. Don't get left behind. Bring your separation tools up to date today with Novex® gels.

Protein Separation Myths:

1. Gel chemistry will always modify my protein

2. NuPAGE® gels take too long to run

3. Precast gels are too expensive

Learn more about Novex® Protein Separation

MYTH: Gel Chemistry Will Always Modify my Protein
FACT: NuPAGE® Gel Chemistry Actually Preserves Your Protein

 

More Neutral pH is the Key

The fundamental shortcoming of the tris-glycine gel system is protein degradation during sample preparation. When using a tris-glycine sample buffer prepared at pH 6.8, samples are heated within the buffer, resulting in a pH decrease due to the negative temperature coefficient of tris-glycine. This ultimately results in protein hydrolysis.

NuPAGE® gel chemistry preserves protein sample integrity by maintaining a higher sample buffer pH (Figure 1). The pH of the NuPAGE® sample buffer does not drop below pH 8 (even when heated to 70°C), so the protein sample does not degrade during sample preparation.

 

Meet the inventor of the NuPAGE® System and learn about preserving your protein during electrophoresis.

  

NuPAGE gel chemistry


Learn more about the NuPAGE® Precast Gel System

 Figure 1. See the difference with NuPAGE® gels. (A) The NuPAGE® gel run using 1X MES buffer. (B) A tris-glycine gel run using tris-glycine/SDS buffer. This figure demonstrates that NuPAGE® gels will produce sharp, intact bands that represent unhydrolyzed protein when compared to tris-glycine gels, particularly evidenced in lanes 3, 5, 7, 8 and 9.

Samples loaded: (1) Mark12™ Unstained Standard, 10 μL; (2) rat liver lysate, 10 μg; (3) lysozyme, 6 μg; (4) Bio-Rad® Broad Range Standard, 1:50 dilution; (5) BSA, 6 μg; (6) MagicMark™ XP Standard, 10 μL; (7) human IgG, 6 μg; (8) human IgM, 6 μg; (9) E. coli lysate, 10 μg; (10) Novex® Sharp Unstained Standard, 10 μL.

MYTH: NuPAGE® Gels Take Too Long to Run
FACT: NuPAGE® Gels Can Be Run in 24 Min at 250V


Standard Run Protocol

To obtain efficient separation, traditional Laemmli gels can take as long as 90 min to run. In contrast, the standard protocol for NuPAGE® gels requires 35 min at 200V. Moreover, our accelerated protocol can typically produce efficient separation in just 24 min at 250V (Figure 2).

 

NuPAGE Gel run timeFigure 2. Accelerate run time without sacrificing performance. NuPAGE® samples loaded: (1) Mark12™ Unstained Standard, 10 μL; (2) rat liver lysate, 10 μg; (3) lysozyme, 6 μg; (4) Bio-Rad® Broad Range Standard, 1:50 dilution; (5) BSA, 6 μg; (6) MagicMark™ XP Standard, 10 μL; (7) human IgG, 6 μg; (8) human IgM, 6 μg; (9) E. coli lysate, 10 μg; (10) Sharp Unstained Standard, 10 μL.


Learn more about the NuPAGE® Precast Gel System

 

 


Observe a Typical Run

MYTH: Precast Gels are too Expensive
FACT: Using Novex® Precast Gels Helps Save Not Only Time and Precious Samples, but Also Money

 

Traditional gel separation hasn't changed much over the last 30 years. Using Novex® precast gels offers the following significant advantages:


  • Less preparation time—you can get your results faster, without the hassle of pouring your own gels, and you can get on with the next step in your workflow
  • Better experiment-to-experiment consistency—our protein gels are subjected to stringent quality control guidelines to minimize variation in your experiments and to help you get more accurate results

Learn more about Novex® Precast Gels

Learn more about our gel casting essentials

 

 

Gel Cost Calculator
Cost Calculator. What does it really cost to prepare and run your own gels vs. using NuPAGE® precast gels, particularly when using them prior to western blotting? Download the NuPAGE® Savings Calculator

Still want to Pour Your Own gels?
Make it fast, simple and easy with Novex® ready to use cassettes.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.