Introduction

The SeeBlue® Plus2 Pre-Stained Standard allows easy visualization of protein molecular weight ranges during electrophoresis and evaluation of western transfer efficiency. The important features of the standard are listed below:

  • Consists of 10 pre-stained protein bands (8 blue and 2 contrasting colors) in the range of 4-250 kDa
  • Suitable for Tris-Glycine, Tricine, and NuPAGE® Novex Gels

Ordering Information

Sku Name Size Price Qty
LC5625 SeeBlue® Pre-stained Protein Standard 500 µL USD 94.75

Protocol

The SeeBlue® Plus2 Pre-Stained Standard is supplied ready-to-use. There is no need to heat or add reducing agent.
Use loading volumes listed below to obtain the best results:

Gel Electrophoresis Blotting
Mini-Gel (1.0 mm thick)10 µl 3 µl
Mini-Gel (1.5 mm thick)20 µl5 µl
Standard Gel (1.0 mm thick) 20 µl 7 µl
Standard Gel (1.5 mm thick) 30 µl 10 µl
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Example

The apparent molecular weights of the protein bands in SeeBlue® Plus2 Pre-Stained Standard in several buffer systems is shown below. The protein bands have different mobilities in various SDS-PAGE buffer systems. For more information on this phenomenon, contact technical service or visit our Web site at www.invitrogen.com.

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Specifications

Contents:                           500 µl of SeeBlue® Plus2 Pre-Stained Standard
Loading Buffer:                 Tris-HCl, Formamide, SDS, Phenol Red
Storage:                            Store at 4°C
Stability:                             4 months at 4°C
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LT059