Organism Type Human The 293 cell line is a permanent line of primary human embryonal kidney transformed by sheared human adenovirus type 5 (Ad 5) DNA. The cells express the transforming gene of adenovirus 5 They are excellent for titrating human adenovirus, see www.atcc.org The GIBCO cells are 293F and 293H. They are pre-adapted to suspension culture in CD 293 or 293 SFM II. Cells which are not pre-adapted will not grow well in these SFM. They would need to be adapted to suspension culture first. The ATCC cells grow adherently, but could probably be adapted to suspension culture. What does the A in 293A stand for? It kind of stands for "adherent" just because the 293A cells (which are just a single cell clone of regular 293s) tend to adhere and form nice flat monolayers in tissue culture dishes. This is why they work so well for plaque assays (regular 293s would be too spindly and would have lots of holes in their monolayer). (P. Welch, 9/02).
Tissues Kidney
Phenotype Either
Primary no
Application Cell Culture / Growth Conditions,Nucleic Acid Purification,Protein Expression,Protein Purification,Stable Cell Transfection,Transfection: Gene Expression,Transfection: Gene Silencing,Transient Transfection,Virus Production

Products

Catalog # Name Size List Price (USD) Qty
10131027 Geneticin® Selective Antibiotic (G418 Sulfate) (50 mg/mL) 100 mL 437.00
10131035 Geneticin® Selective Antibiotic (G418 Sulfate) (50 mg/mL) 20 mL 122.00
10439016 Fetal Bovine Serum, embryonic stem cell-qualified, USDA-approved regions 100 mL 172.88
10687010 Hygromycin B (50 mg/mL) 20 mL 169.00
11058021 Opti-MEM® I Reduced Serum Medium, no phenol red 500 mL 41.70
11095098 MEM 10 x 500 mL 188.00
11514015 PLUS™ Reagent 0.85 mL 214.00
11668019 Lipofectamine® 2000 Transfection Reagent 1.5 mL 528.00
11668027 Lipofectamine® 2000 Transfection Reagent 0.75 mL 316.00
11686029 293 SFM II 1,000 mL 88.00
11811023 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 1 g 113.00
11811031 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 5 g 343.00
11811098 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 25 g 1,408.00
11875101 RPMI 1640 Medium 100 mL 9.55
11913019 CD 293 Medium (1X) 1,000 mL 78.00
11960051 DMEM, high glucose, no glutamine 1,000 mL 34.70
11965092 DMEM, high glucose 500 mL 21.65
11965118 DMEM, high glucose 10 x 500 mL 188.00
11965126 DMEM, high glucose 6 x 1,000 mL 181.00
12100061 DMEM, powder, high glucose 10 L 22.00
12252011 Oligofectamine™ Transfection Reagent 1 mL 392.20
12338018 FreeStyle™ 293 Expression Medium 1,000 mL 101.00
13778075 Lipofectamine® RNAiMAX Transfection Reagent 0.75 mL 393.26
13778100 Lipofectamine® RNAiMAX Transfection Reagent 0.1 mL 65.72
13778150 Lipofectamine® RNAiMAX Transfection Reagent 1.5 mL 648.72
15140148 Penicillin-Streptomycin (10,000 U/mL) 20 mL 9.90
15338100 Lipofectamine® LTX Reagent with PLUS™ Reagent 1 mL 520.00
15338500 Lipofectamine® LTX Reagent 15 mL 7,270.00
15596018 TRIzol® Reagent 200 mL 299.00
18292011 Lipofectin® Transfection Reagent 1 mL 318.00
18324012 Lipofectamine® Transfection Reagent 1 mL 318.00
18324020 Lipofectamine® Transfection Reagent 4 mL 1,064.00
22600050 Opti-MEM® Reduced Serum Medium, powder 10 x 1 L 196.00
25030149 L-Glutamine (200 mM) 20 mL 8.10
31985062 Opti-MEM® I Reduced Serum Medium 100 mL 18.30
K102001 T-REx™ Complete Kit, with pcDNA™4/TO© Vector 1 kit 1,372.00
K278001 Calcium Phosphate Transfection Kit 75 reactions 363.00
K490040 pcDNA™3.1 Directional TOPO® Expression Kit 40 reactions 1,234.00
K493000 ViraPower™ Adenoviral Gateway™ Expression Kit 1 kit 1,024.00
K495000 ViraPower™ Lentiviral Directional TOPO® Expression Kit 1 kit 1,932.00
K650001 Flp-In™ T-REx™ Core Kit 1 kit 1,524.00
K85001 ProBond™ Purification System 6 purifications 489.00
R21001 Blasticidin S HCl, powder 50 mg 172.00
R25001 Zeocin™ Selection Reagent 8 x 1.25 mL 216.00
R70507 293A Cell Line 3 x 106 cells 402.00
V49220 pAd/BLOCK-iT™-DEST RNAi Gateway Vector 6 µg 874.00
V49320 pAd/CMV/V5-DEST™ Gateway® Vector Kit 6 µg 908.00
V79020 pcDNA™3.1 (+) Mammalian Expression Vector 20 µg 548.00
V80020 pcDNA™3.1/myc-His A, B, & C Mammalian Expression Vectors 20 µg 614.00
V90020 pSecTag2 A, B, & C Mammalian Expression Vectors 20 µg 702.00
V96120 pEF6/V5-His A, B, & C Mammalian Expression Vectors 20 µg 614.00

Every Step of the Way, a Wide Range of Cell Health Products

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.