Organism Type Human A small part of the horacic aorta was dissected from human donor and recipient hearts during heart transplantation. The adventitial layer was dissected from the aorta and cells were isolated with the use of collagenase. The cells that attached were cultured in DMEM, supplemented with L-glutamine, 100 U/mL penicillin, 100 ug/mL streptomycin, and 10% fetal bovine serum.
Tissues Aorta
Phenotype Adherent
Primary yes
Application Cell Culture / Growth Conditions

Products

Catalog # Name Size List Price (USD) Qty
11965092 DMEM, high glucose 500 mL 21.65
15070063 Penicillin-Streptomycin (5,000 U/mL) 100 mL 16.60
16000036 Fetal Bovine Serum, certified, US origin 100 mL 107.00
25030081 L-Glutamine (200 mM) 100 mL 23.40
25030149 L-Glutamine (200 mM) 20 mL 8.10

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