Organism Type Primate - Non Human This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region. The line was derived from the CV-1 cell line (ATCC CCL-70) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. The cells contain a single integrated copy of the complete early region of the SV40 genome.
Tissues Kidney
Phenotype Adherent
Primary no
Application Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transient Transfection

Products

Sku Name Size Price Qty
10459014 DMRIE-C Transfection Reagent 1 mL USD 394.00
10566016 DMEM, high glucose, GlutaMAX™ Supplement 500 mL USD 26.40
11514015 PLUS™ Reagent 0.85 mL USD 214.00
11668019 Lipofectamine® 2000 Transfection Reagent 1.5 mL USD 528.00
11668027 Lipofectamine® 2000 Transfection Reagent 0.75 mL USD 316.00
11811031 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 5 g USD 343.00
11965092 DMEM, high glucose 500 mL USD 21.65
15140122 Penicillin-Streptomycin (10,000 U/mL) 100 mL USD 18.91
15140148 Penicillin-Streptomycin (10,000 U/mL) 20 mL USD 9.90
18292011 Lipofectin® Transfection Reagent 1 mL USD 318.00
18324012 Lipofectamine® Transfection Reagent 1 mL USD 318.00
26140079 Fetal Bovine Serum, qualified, US origin 500 mL USD 368.00
31985062 Opti-MEM® I Reduced Serum Medium 100 mL USD 18.30
V00450 pREP4 Mammalian Expression Vector 20 µg USD 506.00
V22120 pcDNA™6/myc-His A, B, & C Mammalian Expression Vectors 20 µg USD 598.00
V66020 pDisplay™ Mammalian Expression Vector 20 µg USD 447.00
V79020 pcDNA™3.1 (+) Mammalian Expression Vector 20 µg USD 548.00
V90020 pSecTag2 A, B, & C Mammalian Expression Vectors 20 µg USD 702.00

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