Organism Type Hamster The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957. The cells require proline in the medium for growth. ATCC CCL-61
Tissues Ovary
Phenotype Adherent
Primary no
Application Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transient Transfection

Recommended Transfection Reagents

  • Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 (51-79% transfection efficiency and 1 fold protein expression improvement over Lipofectamine® 2000)
  • RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX

Products

Catalog # Name Size List Price (USD) Qty
10131027 Geneticin® Selective Antibiotic (G418 Sulfate) (50 mg/mL) 100 mL 437.00
10131035 Geneticin® Selective Antibiotic (G418 Sulfate) (50 mg/mL) 20 mL 122.00
10313021 DMEM, high glucose, pyruvate, no glutamine 500 mL 23.95
10459014 DMRIE-C Transfection Reagent 1 mL 394.00
10687010 Hygromycin B (50 mg/mL) 20 mL 169.00
11140050 MEM Non-Essential Amino Acids Solution (100X) 100 mL 16.60
11140076 MEM Non-Essential Amino Acids Solution (100X) 20 x 100 mL 295.00
11514015 PLUS™ Reagent 0.85 mL 214.00
11668019 Lipofectamine® 2000 Transfection Reagent 1.5 mL 528.00
11668027 Lipofectamine® 2000 Transfection Reagent 0.75 mL 316.00
11765047 Ham's F-12 Nutrient Mix 1,000 mL 38.30
11765054 Ham's F-12 Nutrient Mix 500 mL 23.95
11765062 Ham's F-12 Nutrient Mix 10 x 500 mL 209.00
11765070 Ham's F-12 Nutrient Mix 6 x 1,000 mL 200.00
11811023 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 1 g 113.00
11811031 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 5 g 343.00
11811098 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 25 g 1,408.00
11960044 DMEM, high glucose, no glutamine 500 mL 21.65
11960051 DMEM, high glucose, no glutamine 1,000 mL 34.70
11960069 DMEM, high glucose, no glutamine 10 x 500 mL 188.00
11960077 DMEM, high glucose, no glutamine 6 x 1,000 mL 181.00
11965084 DMEM, high glucose 1,000 mL 34.70
11965092 DMEM, high glucose 500 mL 21.65
11965118 DMEM, high glucose 10 x 500 mL 188.00
11965126 DMEM, high glucose 6 x 1,000 mL 181.00
12052114 CHO-S-SFM II, with hypoxanthine and thymidine 500 mL 50.00
12571048 MEM α, nucleosides 1,000 mL 59.75
12571063 MEM α, nucleosides 500 mL 37.40
12571071 MEM α, nucleosides 10 x 500 mL 325.00
15070063 Penicillin-Streptomycin (5,000 U/mL) 100 mL 16.60
15140122 Penicillin-Streptomycin (10,000 U/mL) 100 mL 18.91
15140148 Penicillin-Streptomycin (10,000 U/mL) 20 mL 9.90
15338100 Lipofectamine® LTX Reagent with PLUS™ Reagent 1 mL 520.00
15338500 Lipofectamine® LTX Reagent 15 mL 7,270.00
15640055 Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture 100 mL 29.00
16000036 Fetal Bovine Serum, certified, US origin 100 mL 132.35
16000044 Fetal Bovine Serum, certified, US origin 500 mL 499.36
16000069 Fetal Bovine Serum, certified, US origin 1,000 mL 964.40
18292011 Lipofectin® Transfection Reagent 1 mL 318.00
18324012 Lipofectamine® Transfection Reagent 1 mL 318.00
18324020 Lipofectamine® Transfection Reagent 4 mL 1,064.00
21127022 Ham's F-12K (Kaighn's) Medium 500 mL 23.95
26400036 Fetal Bovine Serum, dialyzed , US origin 100 mL 189.90
26400044 Fetal Bovine Serum, dialyzed , US origin 500 mL 702.31
31985062 Opti-MEM® I Reduced Serum Medium 100 mL 18.30
K278001 Calcium Phosphate Transfection Kit 75 reactions 363.00
R21001 Blasticidin S HCl, powder 50 mg 172.00
R25001 Zeocin™ Selection Reagent 8 x 1.25 mL 216.00
V04450 pCEP4 Mammalian Expression Vector 20 µg 516.00
V22020 pcDNA™6/V5-His A, B, & C Mammalian Expression Vectors 20 µg 614.00
V79020 pcDNA™3.1 (+) Mammalian Expression Vector 20 µg 548.00
V79520 pcDNA™3.1 (-) Mammalian Expression Vector 20 µg 548.00
V87120 pTracer™-SV40 Mammalian Expression Vector 20 µg 516.00
V88320 pTracer™-CMV/Bsd Mammalian Expression Vector 20 µg 516.00
V88501 pTracer™-CMV2 Kit 1 kit 776.00
V88520 pTracer™-CMV2 Mammalian Expression Vector 20 µg 516.00
V88720 pTracer™-EF A, B, & C Mammalian Expression Vectors 20 µg 518.00
V90020 pSecTag2 A, B, & C Mammalian Expression Vectors 20 µg 702.00

Every Step of the Way, a Wide Range of Cell Health Products

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.