Organism Type Hamster The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957. The cells require proline in the medium for growth. ATCC CCL-61
Tissues Ovary
Phenotype Adherent
Primary no
Application Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transient Transfection

Recommended Transfection Reagents

  • Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 (51-79% transfection efficiency and 1 fold protein expression improvement over Lipofectamine® 2000)
  • RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX

Products

Sku Name Size Price Qty
10131027 Geneticin® Selective Antibiotic (G418 Sulfate), 50 mg/mL Solution 100 mL USD 437.00
10131035 Geneticin® Selective Antibiotic (G418 Sulfate), 50 mg/mL Solution 20 mL USD 122.00
10313021 DMEM, high glucose, pyruvate, no glutamine 500 mL USD 23.95
10459014 DMRIE-C Transfection Reagent 1 mL USD 394.00
10687010 Hygromycin B (50 mg/mL) 20 mL USD 169.00
11140050 100X MEM Non-Essential Amino Acids Solution 100 mL USD 16.60
11140076 100X MEM Non-Essential Amino Acids Solution 20 x 100 mL USD 295.00
11514015 PLUS™ Reagent 0.85 mL USD 214.00
11668019 Lipofectamine® 2000 Transfection Reagent 1.5 mL USD 528.00
11668027 Lipofectamine® 2000 Transfection Reagent 0.75 mL USD 316.00
11765047 Ham's F-12 Nutrient Mix 1,000 mL USD 38.30
11765054 Ham's F-12 Nutrient Mix 500 mL USD 23.95
11765062 Ham's F-12 Nutrient Mix 10 x 500 mL USD 209.00
11765070 Ham's F-12 Nutrient Mix 6 x 1,000 mL USD 200.00
11811023 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 1 g USD 113.00
11811031 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 5 g USD 343.00
11811098 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 25 g USD 1,408.00
11960044 DMEM, high glucose, no glutamine 500 mL USD 21.65
11960051 DMEM, high glucose, no glutamine 1,000 mL USD 34.70
11960069 DMEM, high glucose, no glutamine 10 x 500 mL USD 188.00
11960077 DMEM, high glucose, no glutamine 6 x 1,000 mL USD 181.00
11965084 DMEM, high glucose 1,000 mL USD 34.70
11965092 DMEM, high glucose 500 mL USD 21.65
11965118 DMEM, high glucose 10 x 500 mL USD 188.00
11965126 DMEM, high glucose 6 x 1,000 mL USD 181.00
12052114 CHO-S-SFM II, with hypoxanthine and thymidine 500 mL USD 50.00
12571048 MEM α, nucleosides 1,000 mL USD 59.75
12571063 MEM α, nucleosides 500 mL USD 37.40
12571071 MEM α, nucleosides 10 x 500 mL USD 325.00
15070063 Penicillin-Streptomycin (5,000 U/mL) 100 mL USD 16.60
15140122 Penicillin-Streptomycin (10,000 U/mL) 100 mL USD 18.91
15140148 Penicillin-Streptomycin (10,000 U/mL) 20 mL USD 9.90
15338100 Lipofectamine® LTX Reagent with PLUS™ Reagent 1 mL USD 520.00
15338500 Lipofectamine® LTX Reagent 15 mL USD 7,270.00
15640055 Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture 100 mL USD 29.00
16000036 Fetal Bovine Serum, certified, US origin 100 mL USD 107.00
16000044 Fetal Bovine Serum, certified, US origin 500 mL USD 405.00
16000069 Fetal Bovine Serum, certified, US origin 1,000 mL USD 781.00
18292011 Lipofectin® Transfection Reagent 1 mL USD 318.00
18324012 Lipofectamine® Transfection Reagent 1 mL USD 318.00
18324020 Lipofectamine® Transfection Reagent 4 mL USD 1,064.00
21127022 Ham's F-12K (Kaighn's) Medium 500 mL USD 23.95
26400036 Fetal Bovine Serum, dialyzed , US origin 100 mL USD 147.00
26400044 Fetal Bovine Serum, dialyzed , US origin 500 mL USD 544.00
31985062 Opti-MEM® I Reduced Serum Medium 100 mL USD 18.30
K278001 Calcium Phosphate Transfection Kit 75 reactions USD 363.00
R21001 Blasticidin S HCl, powder 50 mg USD 172.00
R25001 Zeocin™ Selection Reagent 8 x 1.25 mL USD 216.00
V04450 pCEP4 Mammalian Expression Vector 20 µg USD 516.00
V22020 pcDNA™6/V5-His A, B, & C Mammalian Expression Vectors 20 µg USD 614.00
V79020 pcDNA™3.1 (+) Mammalian Expression Vector 20 µg USD 548.00
V79520 pcDNA™3.1 (-) Mammalian Expression Vector 20 µg USD 548.00
V87120 pTracer™-SV40 Mammalian Expression Vector 20 µg USD 516.00
V88320 pTracer™-CMV/Bsd Mammalian Expression Vector 20 µg USD 516.00
V88501 pTracer™-CMV2 Kit 1 kit USD 776.00
V88520 pTracer™-CMV2 Mammalian Expression Vector 20 µg USD 516.00
V88720 pTracer™-EF A, B, & C Mammalian Expression Vectors 20 µg USD 518.00
V90020 pSecTag2 A, B, & C Mammalian Expression Vectors 20 µg USD 702.00

Every Step of the Way, a Wide Range of Cell Health Products

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.