Organism Type Primate - Non Human This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region. The line was derived from the CV-1 cell line (ATCC CCL-70) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. GIBCO brand cell line is a clonal isolate (COS-7L) adapted to serum-free monolayer culture.
Tissues Kidney
Phenotype Adherent
Primary no
Application Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transfection: Gene Silencing,Transient Transfection

Recommended Transfection Reagents

  • Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 (51-79% transfection efficiency and 4 fold protein expression improvement over Lipofectamine® 2000)
  • RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX

Products

Catalog # Name Size List Price (USD) Qty
10313021 DMEM, high glucose, pyruvate, no glutamine 500 mL 23.95
10459014 DMRIE-C Transfection Reagent 1 mL 394.00
10566016 DMEM, high glucose, GlutaMAX™ Supplement 500 mL 26.40
10567014 DMEM, low glucose, GlutaMAX™ Supplement, pyruvate 500 mL 26.40
10687010 Hygromycin B (50 mg/mL) 20 mL 169.00
11058021 Opti-MEM® I Reduced Serum Medium, no phenol red 500 mL 41.70
11140050 MEM Non-Essential Amino Acids Solution (100X) 100 mL 16.60
11360070 Sodium Pyruvate (100 mM) 100 mL 9.30
11514015 PLUS™ Reagent 0.85 mL 214.00
11668019 Lipofectamine® 2000 Transfection Reagent 1.5 mL 528.00
11668027 Lipofectamine® 2000 Transfection Reagent 0.75 mL 316.00
11681020 VP-SFM (1X) 1,000 mL 70.00
11765054 Ham's F-12 Nutrient Mix 500 mL 23.95
11811023 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 1 g 113.00
11875093 RPMI 1640 Medium 500 mL 21.65
11965084 DMEM, high glucose 1,000 mL 34.70
11965092 DMEM, high glucose 500 mL 21.65
11965118 DMEM, high glucose 10 x 500 mL 188.00
11965126 DMEM, high glucose 6 x 1,000 mL 181.00
12100061 DMEM, powder, high glucose 10 L 22.00
12252011 Oligofectamine™ Transfection Reagent 1 mL 392.20
12274015 Gateway® pcDNA™-DEST40 Vector 6 µg 466.00
12500096 DMEM/F-12, powder 50 L 142.00
15140122 Penicillin-Streptomycin (10,000 U/mL) 100 mL 18.91
15140148 Penicillin-Streptomycin (10,000 U/mL) 20 mL 9.90
15338100 Lipofectamine® LTX Reagent with PLUS™ Reagent 1 mL 520.00
15338500 Lipofectamine® LTX Reagent 15 mL 7,270.00
15596018 TRIzol® Reagent 200 mL 299.00
16000036 Fetal Bovine Serum, certified, US origin 100 mL 132.35
16170086 Bovine Serum, New Zealand origin 100 mL 14.00
18292011 Lipofectin® Transfection Reagent 1 mL 318.00
18324012 Lipofectamine® Transfection Reagent 1 mL 318.00
18324020 Lipofectamine® Transfection Reagent 4 mL 1,064.00
25030149 L-Glutamine (200 mM) 20 mL 8.10
26140079 Fetal Bovine Serum, qualified, US origin 500 mL 474.75
31985062 Opti-MEM® I Reduced Serum Medium 100 mL 18.30
K278001 Calcium Phosphate Transfection Kit 75 reactions 363.00
P41050 Electroporation Cuvettes, 0.1 cm 50 each 194.00
P46050 Electroporation Cuvettes, 0.4 cm, red 50⁄bag 204.00
V04450 pCEP4 Mammalian Expression Vector 20 µg 516.00
V26020 pVAX1 Vector 20 µg 506.00
V79020 pcDNA™3.1 (+) Mammalian Expression Vector 20 µg 548.00
V86420 pcDNA™4/HisMax A, B, & C Mammalian Expression Vectors 20 µg 654.00

Every Step of the Way, a Wide Range of Cell Health Products

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