Organism Type Primate - Non Human This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region. The line was derived from the CV-1 cell line (ATCC CCL-70) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. GIBCO brand cell line is a clonal isolate (COS-7L) adapted to serum-free monolayer culture.
Tissues Kidney
Phenotype Adherent
Primary no
Application Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transfection: Gene Silencing,Transient Transfection

Recommended Transfection Reagents

  • Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 (51-79% transfection efficiency and 4 fold protein expression improvement over Lipofectamine® 2000)
  • RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX

Products

Sku Name Size Price Qty
10313021 DMEM, high glucose, pyruvate, no glutamine 500 mL USD 23.95
10459014 DMRIE-C Transfection Reagent 1 mL USD 394.00
10566016 DMEM, high glucose, GlutaMAX™ Supplement 500 mL USD 26.40
10567014 DMEM, low glucose, GlutaMAX™ Supplement, pyruvate 500 mL USD 26.40
10687010 Hygromycin B (50 mg/mL) 20 mL USD 169.00
11058021 Opti-MEM® I Reduced Serum Medium, no phenol red 500 mL USD 41.70
11140050 100X MEM Non-Essential Amino Acids Solution 100 mL USD 16.60
11360070 100 mM Sodium Pyruvate 100 mL USD 9.30
11514015 PLUS™ Reagent 0.85 mL USD 214.00
11668019 Lipofectamine® 2000 Transfection Reagent 1.5 mL USD 528.00
11668027 Lipofectamine® 2000 Transfection Reagent 0.75 mL USD 316.00
11681020 VP-SFM (1X) 1,000 mL USD 70.00
11765054 Ham's F-12 Nutrient Mix 500 mL USD 23.95
11811023 Geneticin® Selective Antibiotic (G418 Sulfate), Powder 1 g USD 113.00
11875093 RPMI 1640 Medium 500 mL USD 21.65
11965084 DMEM, high glucose 1,000 mL USD 34.70
11965092 DMEM, high glucose 500 mL USD 21.65
11965118 DMEM, high glucose 10 x 500 mL USD 188.00
11965126 DMEM, high glucose 6 x 1,000 mL USD 181.00
12100061 DMEM, powder, high glucose 10 L USD 22.00
12252011 Oligofectamine™ Transfection Reagent 1 mL USD 392.20
12274015 Gateway® pcDNA™-DEST40 Vector 6 µg USD 466.00
12500096 DMEM/F-12, powder 50 L USD 142.00
15140122 Penicillin-Streptomycin (10,000 U/mL) 100 mL USD 18.91
15140148 Penicillin-Streptomycin (10,000 U/mL) 20 mL USD 9.90
15338100 Lipofectamine® LTX Reagent with PLUS™ Reagent 1 mL USD 520.00
15338500 Lipofectamine® LTX Reagent 15 mL USD 7,270.00
15596018 TRIzol® Reagent 200 mL USD 299.00
16000036 Fetal Bovine Serum, certified, US origin 100 mL USD 107.00
16170086 Bovine Serum, New Zealand origin 100 mL USD 14.00
18292011 Lipofectin® Transfection Reagent 1 mL USD 318.00
18324012 Lipofectamine® Transfection Reagent 1 mL USD 318.00
18324020 Lipofectamine® Transfection Reagent 4 mL USD 1,064.00
25030149 200 mM L-Glutamine 20 mL USD 8.10
26140079 Fetal Bovine Serum, qualified, US origin 500 mL USD 368.00
31985062 Opti-MEM® I Reduced Serum Medium 100 mL USD 18.30
K278001 Calcium Phosphate Transfection Kit 75 reactions USD 363.00
P41050 Electroporation Cuvettes, 0.1 cm 50 each USD 194.00
P46050 Electroporation Cuvettes, 0.4 cm, red 50⁄bag USD 204.00
V04450 pCEP4 Mammalian Expression Vector 20 µg USD 516.00
V26020 pVAX1 Vector 20 µg USD 506.00
V79020 pcDNA™3.1 (+) Mammalian Expression Vector 20 µg USD 548.00
V86420 pcDNA™4/HisMax A, B, & C Mammalian Expression Vectors 20 µg USD 654.00

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