Organism Type Mouse ATCC CRL-1934 and CRL-11632; pluripotent embryonic stem cells, full name is ES-D3 The cells spontaneously differentiate into embryonic structures in the absence of a feeder layer or conditioned medium. They can be maintained in the undifferentiated state by frequent subculture (every 2 to 3 days) on confluent feeder layers of irradiated (3000 rads) or mitomycin C treated (0.01 mg/ml for 90 minutes) primary mouse embryonic fibroblasts. Medium conditioned by Leukemia Inhibitory Factor (LIF) may also be used to maintain the undifferentiated state. LIF may also be added to ES-D3 cells cultured on feeder layers for additional support of the undifferentiated state.
Tissues Embryo
Phenotype Adherent
Primary no
Application Cell Culture / Growth Conditions,Transient Transfection

Products

Sku Name Size Price Qty
10439016 Fetal Bovine Serum, embryonic stem cell-qualified, USDA-approved regions 100 mL USD 139.00
10439024 Fetal Bovine Serum, embryonic stem cell-qualified, USDA-approved regions 500 mL USD 477.00
10828028 KnockOut™ Serum Replacement 500 mL USD 277.00
11668019 Lipofectamine® 2000 Transfection Reagent 1.5 mL USD 528.00
11668027 Lipofectamine® 2000 Transfection Reagent 0.75 mL USD 316.00
11965084 DMEM, high glucose 1,000 mL USD 34.70
11965092 DMEM, high glucose 500 mL USD 21.65
11965118 DMEM, high glucose 10 x 500 mL USD 188.00
11965126 DMEM, high glucose 6 x 1,000 mL USD 181.00
16141061 Fetal Bovine Serum, embryonic stem cell-qualified, US origin 100 mL USD 147.00
16141079 Fetal Bovine Serum, embryonic stem cell-qualified, US origin 500 mL USD 538.00

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