Organism Type Human The Flp-In T-REx-293 cell line stably expresses the lacZ-Zeocin fusion gene and the Tet repressor, and is designed for use with the Flp-In T-REx System. The cell line expresses the Tet repressor from pcDNA6/TR and contains a single integrated Flp Recombination Target (FRT) site from pFRT/lacZeo as confirmed by Southern blot analysis. This cell line can be used as a host to generate a tetracycline-inducible Flp-In T-REx expression cell line by cotransfecting the pcDNA5/FRT/TO expression vector containing your gene of interest and the Flp recombinase expression plasmid, pOG44.
Tissues Kidney
Phenotype Adherent
Primary no
Application Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression

Products

Sku Name Size Price Qty
10687010 Hygromycin B (50 mg/mL) 20 mL USD 169.00
11668019 Lipofectamine® 2000 Transfection Reagent 1.5 mL USD 528.00
11828029 Gateway® Vector Conversion System with One Shot® ccdB Survival Cells 1 kit USD 510.00
K492000 BLOCK-iT™ Inducible H1 RNAi Entry Vector Kit 20 reactions USD 956.00
K650001 Flp-In™ T-REx™ Core Kit 1 kit USD 1,524.00
R21001 Blasticidin S HCl, powder 50 mg USD 172.00
R25001 Zeocin™ Selection Reagent 8 x 1.25 mL USD 216.00
R25005 Zeocin™ Selection Reagent 50 mL USD 792.00
R78007 Flp-In™ T-REx™ 293 Cell Line 1 mL USD 1,226.00
V652020 pcDNA™5/FRT/TO Vector Kit 20 µg USD 640.00

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