flp-in t-rex 293 Cells
|Organism Type||Human||The Flp-In T-REx-293 cell line stably expresses the lacZ-Zeocin fusion gene and the Tet repressor, and is designed for use with the Flp-In T-REx System. The cell line expresses the Tet repressor from pcDNA6/TR and contains a single integrated Flp Recombination Target (FRT) site from pFRT/lacZeo as confirmed by Southern blot analysis. This cell line can be used as a host to generate a tetracycline-inducible Flp-In T-REx expression cell line by cotransfecting the pcDNA5/FRT/TO expression vector containing your gene of interest and the Flp recombinase expression plasmid, pOG44.|
|Application||Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression|
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