Organism Type Human A subline of the neuroblastoma SK-N-SH line derived from a metastatic bone tumor. Grows as a mixture of floating and adherent cells.
Tissues Brain
Phenotype Either
Primary no
Application Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transient Transfection

Recommended Transfection Reagents

  • Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 ( <30% transfection efficiency and 1 fold protein expression improvement over Lipofectamine® 2000)
  • RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX

Products

Sku Name Size Price Qty
10569010 DMEM, high glucose, GlutaMAX™ Supplement, pyruvate 500 mL USD 26.40
11095080 MEM 500 mL USD 21.65
11765047 Ham's F-12 Nutrient Mix 1,000 mL USD 38.30
11765054 Ham's F-12 Nutrient Mix 500 mL USD 23.95
11765062 Ham's F-12 Nutrient Mix 10 x 500 mL USD 209.00
11765070 Ham's F-12 Nutrient Mix 6 x 1,000 mL USD 200.00
11875085 RPMI 1640 Medium 1,000 mL USD 34.70
11875093 RPMI 1640 Medium 500 mL USD 21.65
11875101 RPMI 1640 Medium 100 mL USD 9.55
11875119 RPMI 1640 Medium 10 x 500 mL USD 188.00
11875127 RPMI 1640 Medium 20 x 100 mL USD 166.00
11875135 RPMI 1640 Medium 6 x 1,000 mL USD 181.00
15290018 Fungizone® Antimycotic 20 mL USD 21.60
17502048 N-2 Supplement (100X) 5 mL USD 70.00
17504044 B-27® Supplement (50X), serum free 10 mL USD 85.00
18292011 Lipofectin® Transfection Reagent 1 mL USD 318.00
18324012 Lipofectamine® Transfection Reagent 1 mL USD 318.00
21103049 Neurobasal® Medium 500 mL USD 58.00
25030149 L-Glutamine (200 mM) 20 mL USD 8.10
26140079 Fetal Bovine Serum, qualified, US origin 500 mL USD 368.00
K278001 Calcium Phosphate Transfection Kit 75 reactions USD 363.00
V102020 pcDNA™4/TO Mammalian Expression Vector 20 µg USD 530.00
V102520 pcDNA™6/TR vector Mammalian Expression Vector 20 µg USD 446.00

Every Step of the Way, a Wide Range of Cell Health Products

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.