Organism Type Rabbit Male New Zealand rabbits (1-2 kg) were anesthetized with 30 mg/kg pentobarbital (Abbott Laboratories, North Chicago, IL), and the thorax and abdomen were opened by a midline incision. The aorta was rapidly removed, and VSMC were isolated. Cells between four and eight passages were plated in 12 or 24 wells or 100-mm plates. Cells were maintained under 5% CO2 in M-199 medium with penicillin, streptomycin, and 10% FBS.
Tissues Aorta
Phenotype Adherent
Primary yes
Application Stable Cell Transfection,Transient Transfection

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