Technical Resources

12491 - Advanced DMEM

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 37.5 0.5
L-Alanine 8.9 Infinity
L-Arginine hydrochloride 84.0 Infinity
L-Asparagine 13.2 Infinity
L-Aspartic acid 13.3 Infinity
L-Cystine 2HCl 63.0 Infinity
L-Glutamic Acid 14.7 Infinity
L-Histidine hydrochloride-H2O 42.0 Infinity
L-Isoleucine 105.0 Infinity
L-Leucine 105.0 Infinity
L-Lysine hydrochloride 146.0 Infinity
L-Methionine 30.0 Infinity
L-Phenylalanine 66.0 Infinity
L-Proline 11.5 Infinity
L-Serine 52.5 Infinity
L-Threonine 95.0 Infinity
L-Tryptophan 16.0 Infinity
L-Tyrosine disodium salt dihydrate 104.0 Infinity
L-Valine 94.0 Infinity
Vitamins
Ascorbic Acid phosphate 2.5 Infinity
Choline chloride 4.0 Infinity
D-Calcium pantothenate 477.0 4.0 0.008385744
Folic Acid 441.0 4.0 0.009070295
Niacinamide 4.0 Infinity
Pyridoxine hydrochloride 4.0 Infinity
Riboflavin 0.4 Infinity
Thiamine hydrochloride 4.0 Infinity
i-Inositol 7.2 Infinity
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 200.0 1.8018018
Ferric Nitrate (Fe(NO3)3"9H2O) 0.1 Infinity
Magnesium Sulfate (MgSO4) (anhyd.) 97.67 Infinity
Potassium Chloride (KCl) 400.0 Infinity
Sodium Bicarbonate (NaHCO3) 3700.0 Infinity
Sodium Chloride (NaCl) 6400.0 Infinity
Sodium Phosphate dibasic (Na2HPO4-H2O) 125.0 Infinity
Proteins
AlbuMAX® II 400.0 Infinity
Human Transferrin (Holo) 7.5 Infinity
Insulin Recombinant Full Chain 10.0 Infinity
Trace Elements
Ammonium Metavanadate 3.0E-4 Infinity
Cupric Sulfate 0.00125 Infinity
Manganous Chloride 5.0E-5 Infinity
Sodium Selenite 0.0050 Infinity
Other Components
D-Glucose (Dextrose) 4500.0 Infinity
Ethanolamine 1.9 Infinity
Glutathione (reduced) 307.0 1.0 0.0032573289
Phenol Red 15.0 Infinity
Sodium Pyruvate 110.0 Infinity

Reference:

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