Technical Resources

32561 - MEM alpha, GlutaMAX(TM), no nucleosides

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 50.0 0.6666667
L-Alanine 89.0 25.0 0.28089887
L-Alanyl-L-Glutamine 203.0 406.0 2.0
L-Arginine 211.0 105.0 0.49763033
L-Asparagine-H2O 132.0 50.0 0.37878788
L-Aspartic acid 133.0 30.0 0.22556391
L-Cysteine hydrochloride 158.0 100.0 0.6329114
L-Cystine 240.0 24.0 0.1
L-Glutamic Acid 147.0 75.0 0.5102041
L-Histidine 155.0 31.0 0.2
L-Isoleucine 131.0 52.4 0.4
L-Leucine 131.0 52.4 0.4
L-Lysine 146.0 58.0 0.39726028
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 32.0 0.19393939
L-Proline 115.0 40.0 0.3478261
L-Serine 105.0 25.0 0.23809524
L-Threonine 119.0 48.0 0.40336135
L-Tryptophan 204.0 10.0 0.04901961
L-Tyrosine 181.0 36.0 0.19889502
L-Valine 117.0 46.0 0.3931624
Vitamins
Ascorbic Acid 176.0 50.0 0.2840909
Biotin 244.0 0.1 4.0983607E-4
Choline chloride 140.0 1.0 0.007142857
D-Calcium pantothenate 477.0 1.0 0.002096436
Folic Acid 441.0 1.0 0.0022675737
Niacinamide 122.0 1.0 0.008196721
Pyridoxal hydrochloride 204.0 1.0 0.004901961
Riboflavin 376.0 0.1 2.6595744E-4
Thiamine hydrochloride 337.0 1.0 0.002967359
Vitamin B12 1355.0 1.36 0.0010036901
i-Inositol 180.0 2.0 0.011111111
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147.0 264.0 1.7959183
Magnesium Sulfate (MgSO4-7H2O) 246.0 200.0 0.8130081
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6800.0 117.24138
Sodium Phosphate monobasic (NaH2PO4-2H2O) 156.0 158.0 1.0128205
Other Components
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Lipoic Acid 206.0 0.2 9.708738E-4
Phenol Red 376.4 10.0 0.026567481
Sodium Pyruvate 110.0 110.0 1.0

Reference:

1. Simonsen, C.C. and Levinson, A.D. 1983. Isolation and expression of an altered mouse dihydrofolate reductase cDNA. Proc. Natl. Acad. Sci. U.S.A. 80:2495-2499.

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