Technical Resources

22400 - RPMI 1640, HEPES

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 10.0 0.13333334
L-Arginine 174.0 200.0 1.1494253
L-Asparagine 132.0 50.0 0.37878788
L-Aspartic acid 133.0 20.0 0.15037593
L-Cystine 2HCl 313.0 65.0 0.20766774
L-Glutamic Acid 147.0 20.0 0.13605443
L-Glutamine 146.0 300.0 2.0547945
L-Histidine 155.0 15.0 0.09677419
L-Hydroxyproline 131.0 20.0 0.15267175
L-Isoleucine 131.0 50.0 0.3816794
L-Leucine 131.0 50.0 0.3816794
L-Lysine hydrochloride 146.0 40.0 0.2739726
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 15.0 0.09090909
L-Proline 115.0 20.0 0.17391305
L-Serine 105.0 30.0 0.2857143
L-Threonine 119.0 20.0 0.16806723
L-Tryptophan 204.0 5.0 0.024509804
L-Tyrosine disodium salt dihydrate 261.0 29.0 0.11111111
L-Valine 117.0 20.0 0.17094018
Vitamins
Biotin 244.0 0.2 8.1967213E-4
Choline chloride 140.0 3.0 0.021428572
D-Calcium pantothenate 477.0 0.25 5.24109E-4
Folic Acid 441.0 1.0 0.0022675737
Niacinamide 122.0 1.0 0.008196721
Para-Aminobenzoic Acid 137.0 1.0 0.00729927
Pyridoxine hydrochloride 206.0 1.0 0.004854369
Riboflavin 376.0 0.2 5.319149E-4
Thiamine hydrochloride 337.0 1.0 0.002967359
Vitamin B12 1355.0 0.0050 3.690037E-6
i-Inositol 180.0 35.0 0.19444445
Inorganic Salts
Calcium nitrate (Ca(NO3)2 4H2O) 236.0 100.0 0.42372882
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 48.84 0.407
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2000.0 23.809525
Sodium Chloride (NaCl) 58.0 5300.0 91.37931
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142.0 800.0 5.633803
Other Components
D-Glucose (Dextrose) 180.0 2000.0 11.111111
Glutathione (reduced) 307.0 1.0 0.0032573289
HEPES 238.0 5958.0 25.033613
Phenol Red 376.4 5.0 0.013283741

Reference:

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