Technical Resources

42430 - DMEM, high glucose, HEPES

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 30.0 0.4
L-Arginine hydrochloride 211.0 84.0 0.39810428
L-Cystine 2HCl 313.0 63.0 0.20127796
L-Glutamine 146.0 580.0 3.9726028
L-Histidine hydrochloride-H2O 210.0 42.0 0.2
L-Isoleucine 131.0 105.0 0.8015267
L-Leucine 131.0 105.0 0.8015267
L-Lysine hydrochloride 183.0 146.0 0.7978142
L-Methionine 149.0 30.0 0.20134228
L-Phenylalanine 165.0 66.0 0.4
L-Serine 105.0 42.0 0.4
L-Threonine 119.0 95.0 0.79831934
L-Tryptophan 204.0 16.0 0.078431375
L-Tyrosine 181.0 72.0 0.39779004
L-Valine 117.0 94.0 0.8034188
Vitamins
Choline chloride 140.0 4.0 0.028571429
D-Calcium pantothenate 477.0 4.0 0.008385744
Folic Acid 441.0 4.0 0.009070295
Niacinamide 122.0 4.0 0.032786883
Pyridoxine hydrochloride 204.0 4.0 0.019607844
Riboflavin 376.0 0.4 0.0010638298
Thiamine hydrochloride 337.0 4.0 0.011869436
i-Inositol 180.0 7.2 0.04
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147.0 264.0 1.7959183
Ferric Nitrate (Fe(NO3)3"9H2O) 404.0 0.1 2.4752476E-4
Magnesium Sulfate (MgSO4-7H2O) 246.0 200.0 0.8130081
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 3700.0 44.04762
Sodium Chloride (NaCl) 58.0 3500.0 60.344826
Sodium Phosphate monobasic (NaH2PO4-2H2O) 154.0 141.0 0.91558444
Other Components
D-Glucose (Dextrose) 180.0 4500.0 25.0
HEPES 238.0 5958.0 25.033613
Phenol Red 376.4 15.0 0.039851222

Reference:

1. Dulbecco, R. and Freeman, G. (1959) Virology 8:396. 

Related Products:

11995 - DMEM, high glucose, pyruvate
Cat No: 11995040 , 11995065 , 11995073 , 11995081 , 11995115 , 11995123

12430 - DMEM, high glucose, HEPES
Cat No: 12430047 , 12430054 , 12430062 , 12430104 , 12430112 , 12430120

21063 - DMEM, high glucose, HEPES, no phenol red
Cat No: 21063029 , 21063045

11971 - DMEM, high glucose, no phosphates
Cat No: 11971025

41966 - DMEM, high glucose, pyruvate
Cat No: 41966029 , 41966161