Technical Resources

32404 - RPMI 1640, no glutamine, no phenol red

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 10.0 0.13333334
L-Arginine 174.0 200.0 1.1494253
L-Asparagine 132.0 50.0 0.37878788
L-Aspartic acid 133.0 20.0 0.15037593
L-Cystine 240.0 20.0 0.083333336
L-Glutamic Acid 147.0 20.0 0.13605443
L-Histidine 155.0 15.0 0.09677419
L-Hydroxyproline 131.0 20.0 0.15267175
L-Isoleucine 131.0 50.0 0.3816794
L-Leucine 131.0 50.0 0.3816794
L-Lysine hydrochloride 146.0 40.0 0.2739726
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 15.0 0.09090909
L-Proline 115.0 20.0 0.17391305
L-Serine 105.0 30.0 0.2857143
L-Threonine 119.0 20.0 0.16806723
L-Tryptophan 204.0 5.0 0.024509804
L-Tyrosine 181.0 20.0 0.110497236
L-Valine 117.0 20.0 0.17094018
Vitamins
Biotin 244.0 0.2 8.1967213E-4
Choline chloride 140.0 3.0 0.021428572
D-Calcium pantothenate 477.0 0.25 5.24109E-4
Folic Acid 441.0 1.0 0.0022675737
Niacinamide 122.0 1.0 0.008196721
Para-Aminobenzoic Acid 137.0 1.0 0.00729927
Pyridoxine hydrochloride 206.0 1.0 0.004854369
Riboflavin 376.0 0.2 5.319149E-4
Thiamine hydrochloride 337.0 1.0 0.002967359
Vitamin B12 1355.0 0.0050 3.690037E-6
i-Inositol 180.0 35.0 0.19444445
Inorganic Salts
Calcium nitrate (Ca(NO3)2 4H2O) 236.0 100.0 0.42372882
Magnesium Sulfate (MgSO4-7H2O) 246.0 100.0 0.40650406
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2000.0 23.809525
Sodium Chloride (NaCl) 58.0 6000.0 103.44827
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142.0 800.0 5.633803
Other Components
D-Glucose (Dextrose) 180.0 2000.0 11.111111
Glutathione (reduced) 307.0 1.0 0.0032573289

Reference:

1. Moore, G.E., Gerner, R.E. and Franklin, H.A. (1967) J.A.M.A., 199:519.

2. Mossinger, J. (1991) Parasitol., 103:85 (Nematodes, Litomosoides carinii).

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