Technical Resources

32360 - MEM, HEPES, no glutamine

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
L-Arginine hydrochloride 211.0 126.0 0.5971564
L-Cystine 240.0 24.0 0.1
L-Histidine hydrochloride-H2O 210.0 42.0 0.2
L-Isoleucine 131.0 52.0 0.39694658
L-Leucine 131.0 52.0 0.39694658
L-Lysine hydrochloride 183.0 73.0 0.3989071
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 32.0 0.19393939
L-Threonine 119.0 48.0 0.40336135
L-Tryptophan 204.0 10.0 0.04901961
L-Tyrosine 181.0 36.0 0.19889502
L-Valine 117.0 46.0 0.3931624
Vitamins
Choline chloride 140.0 1.0 0.007142857
D-Calcium pantothenate 477.0 1.0 0.002096436
Folic Acid 441.0 1.0 0.0022675737
Niacinamide 122.0 1.0 0.008196721
Pyridoxal hydrochloride 204.0 1.0 0.004901961
Riboflavin 376.0 0.1 2.6595744E-4
Thiamine hydrochloride 337.0 1.0 0.002967359
i-Inositol 180.0 2.0 0.011111111
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147.0 264.0 1.7959183
Magnesium Sulfate (MgSO4-7H2O) 246.0 200.0 0.8130081
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6300.0 108.62069
Sodium Phosphate monobasic (NaH2PO4-2H2O) 156.0 158.0 1.0128205
Other Components
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
HEPES 238.0 5960.0 25.042017
Phenol Red 376.4 10.0 0.026567481

Reference:

1. Eagle, H. (1959) Science 130:432.

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