Technical Resources

41010 - Basal Medium Eagle (BME), no Glutamine

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
L-Arginine hydrochloride 211.0 21.0 0.09952607
L-Cystine 240.0 12.0 0.05
L-Histidine 155.0 8.0 0.051612902
L-Isoleucine 131.0 26.0 0.19847329
L-Leucine 131.0 26.0 0.19847329
L-Lysine hydrochloride 183.0 29.0 0.15846995
L-Methionine 149.0 7.5 0.05033557
L-Phenylalanine 165.0 16.5 0.1
L-Threonine 119.0 24.0 0.20168068
L-Tryptophan 204.0 4.0 0.019607844
L-Tyrosine 181.0 18.0 0.09944751
L-Valine 117.0 23.5 0.2008547
Vitamins
Biotin 244.0 1.0 0.0040983604
Choline chloride 140.0 1.0 0.007142857
D-Calcium pantothenate 477.0 1.0 0.002096436
Folic Acid 441.0 1.0 0.0022675737
Nicotinamide 122.0 1.0 0.008196721
Pyridoxal hydrochloride 204.0 1.0 0.004901961
Riboflavin 376.0 0.1 2.6595744E-4
Thiamine hydrochloride 337.0 1.0 0.002967359
i-Inositol 180.0 1.8 0.01
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147.0 264.0 1.7959183
Magnesium Sulfate (MgSO4-7H2O) 246.0 200.0 0.8130081
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6800.0 117.24138
Sodium Phosphate monobasic (NaH2PO4-2H2O) 156.0 158.0 1.0128205
Other Components
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Phenol Red 376.4 10.0 0.026567481

Reference:

1. Eagle, H. (1965) Proc. Exp. Biol. Med., 89:362.

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21010 - Basal Medium Eagle (BME), no Glutamine
Cat No: 21010046