Technical Resources

21540 - CMRL, 10x, no Glutamine

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 500.0 6.6666665
Hydroxy L-proline 131.0 100.0 0.7633588
L-Alanine 89.0 250.0 2.8089888
L-Arginine hydrochloride 211.0 700.0 3.3175356
L-Aspartic acid 133.0 300.0 2.255639
L-Cysteine 121.0 1998.8 16.519009
L-Cystine 240.0 200.0 0.8333333
L-Glutamic Acid 147.0 750.0 5.102041
L-Histidine hydrochloride-H2O 210.0 200.0 0.95238096
L-Isoleucine 131.0 200.0 1.5267175
L-Leucine 131.0 600.0 4.5801525
L-Lysine hydrochloride 183.0 700.0 3.8251367
L-Methionine 149.0 150.0 1.0067114
L-Phenylalanine 165.0 250.0 1.5151515
L-Proline 115.0 400.0 3.4782608
L-Serine 105.0 250.0 2.3809524
L-Threonine 119.0 300.0 2.5210085
L-Tryptophan 204.0 100.0 0.49019608
L-Tyrosine 181.0 400.0 2.2099447
L-Valine 117.0 250.0 2.1367521
Vitamins
Ascorbic Acid 176.0 500.0 2.840909
Biotin 244.0 0.1 4.0983607E-4
Cholesterol 387.0 2.0 0.005167959
Choline chloride 140.0 5.0 0.035714287
D-Calcium pantothenate 477.0 0.1 2.0964361E-4
Folic Acid 441.0 0.1 2.2675737E-4
Niacinamide 122.0 0.25 0.0020491802
Nicotinic acid (Niacin) 123.0 0.25 0.0020325202
Para-Aminobenzoic Acid 137.0 0.5 0.003649635
Pyridoxine hydrochloride 206.0 0.25 0.0012135922
Riboflavin 376.0 0.1 2.6595744E-4
Thiamine hydrochloride 337.0 0.1 2.967359E-4
i-Inositol 180.0 0.5 0.0027777778
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147.0 2000.0 13.605442
Magnesium Sulfate (MgSO4-7H2O) 246.0 2000.0 8.130081
Potassium Chloride (KCl) 75.0 4000.0 53.333332
Sodium Chloride (NaCl) 58.0 6799.0 117.22414
Sodium Phosphate monobasic (NaH2PO4-2H2O) 156.0 1580.0 10.128205
Other Components
2'Deoxyadenosine 251.0 100.0 0.3984064
2'Deoxycytidine 227.0 100.0 0.44052863
2'Deoxyguanosine 267.0 100.0 0.37453184
5-Methyl-deoxycytidine 225.0 1.0 0.0044444446
Co-carboxylase 461.0 10.0 0.021691974
Coenzyme A 768.0 25.0 0.032552082
D-Glucose (Dextrose) 180.0 10000.0 55.555557
Diphosphopyridine nucleotide (NAD) 663.0 70.0 0.105580695
FAD (flavin adenine dinucleotide) 786.0 10.0 0.012722646
Glutathione (reduced) 307.0 100.0 0.3257329
Phenol Red 376.4 200.0 0.53134966
Sodium acetate-3H2O 136.0 830.0 6.102941
Sodium glucuronate-H2O 236.0 40.0 0.16949153
Thymidine 242.0 100.0 0.41322315
Triphosphopyridine Nucleotide (NADP) 743.0 10.0 0.01345895
Tween 80® 50.0 Infinity
Uridine 5'- triphosphate 484.0 10.0 0.020661157

Reference:

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