Technical Resources

31330 - DMEM/F-12, HEPES

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 18.75 0.25
L-Alanine 89.0 4.45 0.049999997
L-Arginine hydrochloride 211.0 147.5 0.69905216
L-Asparagine-H2O 150.0 7.5 0.05
L-Aspartic acid 133.0 6.65 0.05
L-Cysteine hydrochloride-H2O 176.0 17.56 0.09977272
L-Cystine 2HCl 313.0 31.29 0.09996805
L-Glutamic Acid 147.0 7.35 0.05
L-Glutamine 146.0 365.0 2.5
L-Histidine hydrochloride-H2O 210.0 31.48 0.14990476
L-Isoleucine 131.0 54.47 0.41580153
L-Leucine 131.0 59.05 0.45076334
L-Lysine hydrochloride 183.0 91.25 0.4986339
L-Methionine 149.0 17.24 0.11570469
L-Phenylalanine 165.0 35.48 0.2150303
L-Proline 115.0 17.25 0.15
L-Serine 105.0 26.25 0.25
L-Threonine 119.0 53.45 0.44915968
L-Tryptophan 204.0 9.02 0.04421569
L-Tyrosine disodium salt dihydrate 261.0 55.79 0.21375479
L-Valine 117.0 52.85 0.4517094
Vitamins
Biotin 244.0 0.0035 1.4344263E-5
Choline chloride 140.0 8.98 0.06414285
D-Calcium pantothenate 477.0 2.24 0.0046960167
Folic Acid 441.0 2.65 0.0060090707
Niacinamide 122.0 2.02 0.016557377
Pyridoxine hydrochloride 206.0 2.0 0.009708738
Riboflavin 376.0 0.219 5.824468E-4
Thiamine hydrochloride 337.0 2.17 0.0064391694
Vitamin B12 1355.0 0.68 5.0184503E-4
i-Inositol 180.0 12.6 0.07
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 116.6 1.0504504
Cupric sulfate (CuSO4-5H2O) 250.0 0.0013 5.2E-6
Ferric Nitrate (Fe(NO3)3"9H2O) 404.0 0.05 1.2376238E-4
Ferrous Sulfate (FeSO4 7H2O) 278.0 0.417 0.0015
Magnesium Chloride (MgCl2-6H2O) 203.0 61.0 0.3004926
Magnesium Sulfate (MgSO4-7H2O) 246.0 100.0 0.40650406
Potassium Chloride (KCl) 75.0 311.8 4.1573334
Sodium Bicarbonate (NaHCO3) 84.0 1200.0 14.285714
Sodium Chloride (NaCl) 58.0 6995.5 120.61207
Sodium Phosphate dibasic (Na2HPO4-7H2O) 268.0 134.0 0.5
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 62.5 0.45289856
Zinc sulfate (ZnSO4-7H2O) 288.0 0.432 0.0015
Other Components
D-Glucose (Dextrose) 180.0 3151.0 17.505556
HEPES 238.0 3574.5 15.018908
Hypoxanthine Na 159.0 2.39 0.015031448
Linoleic Acid 280.0 0.042 1.4999999E-4
Lipoic Acid 206.0 0.105 5.097087E-4
Phenol Red 376.4 8.1 0.021519661
Putrescine 2HCl 161.0 0.081 5.031056E-4
Sodium Pyruvate 110.0 55.0 0.5
Thymidine 242.0 0.365 0.0015082645

Reference:

1. Dulbecco, R. and Freeman, G., (1959) Plaque formation by the polyoma virus. Virology 8:396.

2. Ham, R.G., (1965) Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.

3. Morton, H.J. (1970) A survey of commercially available tissue culture media. In Vitro 6(2):89.

4. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185.

Related Products:

11330 - DMEM/F-12, HEPES
Cat No: 11330032 , 11330057 , 11330099 , 11330107