Technical Resources

12634 - Advanced D-MEM/F-12

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 18.75 0.25
L-Alanine 89.0 4.45 0.049999997
L-Arginine hydrochloride 211.0 147.5 0.69905216
L-Asparagine-H2O 150.0 7.5 0.05
L-Aspartic acid 133.0 6.65 0.05
L-Cysteine hydrochloride-H2O 176.0 17.56 0.09977272
L-Cystine 2HCl 313.0 31.29 0.09996805
L-Glutamic Acid 147.0 7.35 0.05
L-Histidine hydrochloride-H2O 210.0 31.48 0.14990476
L-Isoleucine 131.0 54.47 0.41580153
L-Leucine 131.0 59.05 0.45076334
L-Lysine hydrochloride 183.0 91.25 0.4986339
L-Methionine 149.0 17.24 0.11570469
L-Phenylalanine 165.0 35.48 0.2150303
L-Proline 115.0 17.25 0.15
L-Serine 105.0 26.25 0.25
L-Threonine 119.0 53.45 0.44915968
L-Tryptophan 204.0 9.02 0.04421569
L-Tyrosine disodium salt dihydrate 261.0 55.79 0.21375479
L-Valine 117.0 52.85 0.4517094
Vitamins
Ascorbic Acid phosphate 289.54 2.5 0.008634386
Biotin 244.0 0.0035 1.4344263E-5
Choline chloride 140.0 8.98 0.06414285
D-Calcium pantothenate 477.0 2.24 0.0046960167
Folic Acid 441.0 2.65 0.0060090707
Niacinamide 122.0 2.02 0.016557377
Pyridoxine hydrochloride 206.0 2.0 0.009708738
Riboflavin 376.0 0.219 5.824468E-4
Thiamine hydrochloride 337.0 2.17 0.0064391694
Vitamin B12 1355.0 0.68 5.0184503E-4
i-Inositol 180.0 12.6 0.07
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 116.6 1.0504504
Cupric sulfate (CuSO4-5H2O) 250.0 0.0013 5.2E-6
Ferric Nitrate (Fe(NO3)3"9H2O) 404.0 0.05 1.2376238E-4
Ferric sulfate (FeSO4-7H2O) 278.0 0.417 0.0015
Magnesium Chloride (anhydrous) 95.0 28.64 0.30147368
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 48.84 0.407
Potassium Chloride (KCl) 75.0 311.8 4.1573334
Sodium Bicarbonate (NaHCO3) 84.0 2438.0 29.02381
Sodium Chloride (NaCl) 58.0 6995.5 120.61207
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142.0 71.02 0.50014085
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 62.5 0.45289856
Zinc sulfate (ZnSO4-7H2O) 288.0 0.864 0.0030
Proteins
AlbuMAX® II 400.0 Infinity
Human Transferrin (Holo) 7.5 Infinity
Insulin Recombinant Full Chain 10.0 Infinity
Reducing Agents
Glutathione, monosodium 307.0 1.0 0.0032573289
Trace Elements
Ammonium Metavanadate 116.98 3.0E-4 2.564541E-6
Manganous Chloride 198.0 5.0E-5 2.5252524E-7
Sodium Selenite 173.0 0.0050 2.8901733E-5
Other Components
D-Glucose (Dextrose) 180.0 3151.0 17.505556
Ethanolamine 97.54 1.9 0.019479187
Hypoxanthine Na 159.0 2.39 0.015031448
Linoleic Acid 280.0 0.042 1.4999999E-4
Lipoic Acid 206.0 0.105 5.097087E-4
Phenol Red 376.4 8.1 0.021519661
Putrescine 2HCl 161.0 0.081 5.031056E-4
Sodium Pyruvate 110.0 110.0 1.0
Thymidine 242.0 0.365 0.0015082645

Reference:

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