Technical Resources

31415 - Leibovitz's L-15

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 200.0 2.6666667
L-Alanine 89.0 225.0 2.52809
L-Alanyl-L-Glutamine 217.0 446.0 2.0552995
L-Arginine 174.0 500.0 2.8735633
L-Asparagine 132.0 250.0 1.8939394
L-Cysteine 121.0 120.0 0.9917355
L-Histidine 155.0 250.0 1.6129032
L-Isoleucine 131.0 250.0 1.908397
L-Leucine 131.0 125.0 0.9541985
L-Lysine 146.0 75.0 0.51369864
L-Methionine 149.0 75.0 0.5033557
L-Phenylalanine 165.0 125.0 0.75757575
L-Serine 105.0 200.0 1.9047619
L-Threonine 119.0 300.0 2.5210085
L-Tryptophan 204.0 20.0 0.09803922
L-Tyrosine 181.0 300.0 1.6574585
L-Valine 117.0 100.0 0.85470086
Vitamins
Choline chloride 140.0 1.0 0.007142857
D-Calcium pantothenate 477.0 1.0 0.002096436
Folic Acid 441.0 1.0 0.0022675737
Niacinamide 122.0 1.0 0.008196721
Pyridoxine hydrochloride 206.0 1.0 0.004854369
Riboflavin 5'-phosphate Na 478.0 0.1 2.0920503E-4
Thiamine monophosphate 442.0 1.0 0.0022624435
i-Inositol 180.0 2.0 0.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 140.0 1.2612612
Magnesium Chloride (anhydrous) 95.0 93.7 0.9863158
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 97.67 0.8139166
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Potassium Phosphate monobasic (KH2PO4) 136.0 60.0 0.44117647
Sodium Chloride (NaCl) 58.0 8000.0 137.93103
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142.0 190.0 1.3380282
Other Components
D+ Galactose 180.0 900.0 5.0
Phenol Red 376.4 10.0 0.026567481
Sodium Pyruvate 110.0 550.0 5.0

Reference:

1. Beall, P.T., Karnaky, K.J., Garretson, L.T. et al. (1983) Biochem. Biophys. Acta 763:19 (rat jejumum).

2. Chinchar, G.D. and Sinclair, J.H. (1978) J. Cell. Physiol. 96:333 (amphibian, frog, ICR, 2A).

3. Flamme, I., Albach, K., Muller, S. et al. (1991) Anat. Record, 229:427 (chick embryos).

4. Foxworthy, P.S. and Eacho, P.I. (1986) Toxicol. Ltrs., 30:189 (Rat hepatocytes).

5. Grothe, C., Otto, D. and Unsicker, K. (1989) Neuroscience 31:649 (Rat embryonic E18 septal neurons).

6. Leibovitz, A. (1963) Am. J. Hygiene 78:173.

7. Matsuda, M. (1983) Develop. Biol., 95:219. (Amphibian [newt] neural plates).

8. Middlebrooks, B.L., Mak, P.C. and Ellender, R.D. (1980) Proc. Soc. Exper. Biol. Med., 165:123 (fish fibroblast).

9. Powell, R.L., Dodson, M.V. and Cloud, J.G. (1989) J. Exper. Zool., 50:333 (Fish Satellite cells, mygenic stem cells).

10. Saad, a.H. and El Ridi, R. (1988) Vet. Immunol Imunopath., 18:369 (reptile [lizard] spleen cells).

11. Tate, J.M., Oberpriller, J.O. and Oberpriller, J.C. (1989) Tissue and Cell, 21:335 (Newt cardiac monocytes).

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11415 - Leibovitz's L-15
Cat No: 11415049 , 11415056 , 11415064 , 11415114