Technical Resources

NEUROBASAL™ Medium (1X) liquid

Neurobasal™ Media are basal media formulated to meet the neuronal cells special requirements.

They allow for long-term maintenance of the normal phenotype and growth of neuronal cells, and maintain pure populations of neuronal cells without the need of an astrocyte feeder layer.

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 30.0 0.4
L-Alanine 89.0 2.0 0.02247191
L-Arginine hydrochloride 211.0 84.0 0.39810428
L-Asparagine-H2O 150.0 0.83 0.0055333334
L-Cysteine 121.0 31.5 0.2603306
L-Histidine hydrochloride-H2O 210.0 42.0 0.2
L-Isoleucine 131.0 105.0 0.8015267
L-Leucine 131.0 105.0 0.8015267
L-Lysine hydrochloride 183.0 146.0 0.7978142
L-Methionine 149.0 30.0 0.20134228
L-Phenylalanine 165.0 66.0 0.4
L-Proline 115.0 7.76 0.06747826
L-Serine 105.0 42.0 0.4
L-Threonine 119.0 95.0 0.79831934
L-Tryptophan 204.0 16.0 0.078431375
L-Tyrosine 181.0 72.0 0.39779004
L-Valine 117.0 94.0 0.8034188
Vitamins
Choline chloride 140.0 4.0 0.028571429
D-Calcium pantothenate 477.0 4.0 0.008385744
Folic Acid 441.0 4.0 0.009070295
Niacinamide 122.0 4.0 0.032786883
Pyridoxine hydrochloride 204.0 4.0 0.019607844
Riboflavin 376.0 0.4 0.0010638298
Thiamine hydrochloride 337.0 4.0 0.011869436
Vitamin B12 1355.0 0.0068 5.0184503E-6
i-Inositol 180.0 7.2 0.04
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 200.0 1.8018018
Ferric Nitrate (Fe(NO3)3"9H2O) 404.0 0.1 2.4752476E-4
Magnesium Chloride (anhydrous) 95.0 77.3 0.8136842
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 3000.0 51.724136
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 125.0 0.9057971
Zinc sulfate (ZnSO4-7H2O) 288.0 0.194 6.736111E-4
Other Components
D-Glucose (Dextrose) 180.0 4500.0 25.0
HEPES 238.0 2600.0 10.92437
Phenol Red 376.4 8.1 0.021519661
Sodium Pyruvate 110.0 25.0 0.22727273

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