Technical Resources

Medium 154

Without Calcium.

A sterile, liquid medium for the culture of human epidermal keratinocytes. Medium 154CF is Medium 154 prepared without calcium chloride. Calcium chloride is provided as a separate component with each bottle of medium. This basal medium requires the addition of HKGS (S-001-5) or HKGS Kit (S-001-K) prior to use. Below is the composition list. The concentrations are proprietary.

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 confidential n/a
L-Alanine 89.0 confidential n/a
L-Arginine hydrochloride 211.0 confidential n/a
L-Asparagine-H2O 150.0 confidential n/a
L-Aspartic acid 133.0 confidential n/a
L-Cysteine 121.0 confidential n/a
L-Glutamic Acid 147.0 confidential n/a
L-Glutamine 146.0 confidential n/a
L-Histidine hydrochloride-H2O 210.0 confidential n/a
L-Isoleucine 131.0 confidential n/a
L-Leucine 131.0 confidential n/a
L-Lysine hydrochloride 183.0 confidential n/a
L-Methionine 149.0 confidential n/a
L-Phenylalanine 165.0 confidential n/a
L-Proline 115.0 confidential n/a
L-Serine 105.0 confidential n/a
L-Threonine 119.0 confidential n/a
L-Tryptophan 204.0 confidential n/a
L-Tyrosine 181.0 confidential n/a
L-Valine 117.0 confidential n/a
Vitamins
Choline chloride 140.0 confidential n/a
D-Pantothenic Acid 238.0 confidential n/a
Folic Acid 441.0 confidential n/a
Myo-Inositol 180.0 confidential n/a
Niacinamide 122.0 confidential n/a
Pyridoxal hydrochloride 206.0 confidential n/a
Riboflavin 376.0 confidential n/a
Thiamine hydrochloride 337.0 confidential n/a
Vitamin B12 1355.0 confidential n/a
d-Biotin 244.0 confidential n/a
Inorganic Salts
Ammonium Metavanadate NaVO3 117.0 confidential n/a
Ammonium Molybdate ((NH4)6Mo7O24-4H2O) 1236.0 confidential n/a
Cupric sulfate (CuSO4-5H2O) 250.0 confidential n/a
Ferric sulfate (FeSO4-7H2O) 278.0 confidential n/a
Magnesium Chloride (MgCl2-6H2O) 203.0 confidential n/a
Manganese Sulfate (MnSO4-H20) 169.0 confidential n/a
Nickelous Chloride NiCl2 6H2O 238.0 confidential n/a
Potassium Chloride (KCl) 75.0 confidential n/a
Sodium Acetate (NaCH3COO.3H2O) 136.0 confidential n/a
Sodium Bicarbonate (NaHCO3) 84.0 confidential n/a
Sodium Chloride (NaCl) 58.0 confidential n/a
Sodium Meta Silicate Na2SiO3 9H2O 284.0 confidential n/a
Sodium Phosphate dibasic (Na2HPO4-7H2O) 268.0 confidential n/a
Sodium Selenite (Na2SeO3) 173.0 confidential n/a
Tin Chloride (SnCl2.2H2O) 226.0 confidential n/a
Zinc sulfate (ZnSO4-7H2O) 288.0 confidential n/a
Other Components
Adenine.HCl 172.0 confidential n/a
D-Glucose (Dextrose) 180.0 confidential n/a
DL-alpha-Lipoic Acid 206.0 confidential n/a
Ethanolamine 61.0 confidential n/a
HEPES 238.0 confidential n/a
O-Phosphorylethanolamine 141.0 confidential n/a
Phenol Red 376.4 confidential n/a
Putrescine 2HCl 161.0 confidential n/a
Sodium Pyruvate 110.0 confidential n/a
Thymidine 242.0 confidential n/a

Reference:

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